REtools: a laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance

BMC Bioinformatics
Patrick MartinPhilippe Pognonec

Abstract

Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance. Several manufacturers propose on-line sites that assist scientists in their restriction enzyme work, however, none of these sites meet all the actual needs of laboratory workers, and they do not take into account the enzymes actually present in one's own laboratory. Using FileMaker Pro, we developed a stand-alone application which can run on both PCs and Macintoshes. We called it REtools, for Restriction Enzyme tools. This program, which references all currently known enzymes (>3500), permits the creation and update of a personalized list of restriction enzymes actually available in one's own laboratory. Upon opening the program, scientists will be presented with a user friendly interface that will direct them to different menus, each one corresponding to different situations that restriction enzyme users commonly encounter. We particularly emphasized the ease of use to make REtools a solution that laboratory members would actually want to use. REtools, a user friendly and easily customized pr...Continue Reading

References

Jul 25, 2000·BioTechniques·A ZaldumbideP Pognonec
Mar 17, 2001·BMC Genetics·K E Boulukos, P Pognonec
Jun 26, 2003·Nucleic Acids Research·Tamas VinczeRichard J Roberts

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Citations

Jul 29, 2009·Biotechnology Progress·Xiang ChenMing Chen
Jun 18, 2009·Chembiochem : a European Journal of Chemical Biology·Douglas D YoungAlexander Deiters
Feb 9, 2012·Molecular BioSystems·Ting WeiJue Liu

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Methods Mentioned

BETA
PCR

Software Mentioned

FileMaker Pro
Promega
Protos
FileMaker
EnzymeX
AllREnar
REtools
MacOSX10
NEB
NEBCutter

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