Retracing Schwann Cell Developmental Transitions in Embryonic Dissociated DRG/Schwann Cell Cocultures in Mice.

Frontiers in Cellular Neuroscience
Venkat Krishnan SundaramJ. Grenier

Abstract

Embryonic Dissociated Dorsal Root Ganglia (DRG) cultures are often used to investigate the role of novel molecular pathways or drugs in Schwann cell development and myelination. These cultures largely recapitulate the order of cellular and molecular events that occur in Schwann cells of embryonic nerves. However, the timing of Schwann cell developmental transitions, notably the transition from Schwann Cell Precursors (SCP) to immature Schwann cells (iSC) and then to myelinating Schwann cells, has not been estimated so far in this culture system. In this study, we determined the expression profiles of Schwann cell developmental genes during the first week of culture and then compared our data to the expression profiles of these genes in developing spinal nerves. This helped in identifying that SCP transition into iSC between the 5th and 7th day in vitro. Furthermore, we also investigated the transition of immature cells into pro-myelinating and myelinating Schwann cells upon the induction of myelination in vitro. Our results suggest that Schwann cell differentiation beyond the immature stage can be observed as early as 4 days post the induction of myelination in cocultures. Finally, we compared the myelinating potential of cocul...Continue Reading

References

Jul 26, 1996·Science·M JaegleD Meijer
May 26, 1999·Journal of Neuroscience Research·Z DongK R Jessen
Mar 21, 2000·Genesis : the Journal of Genetics and Development·O VoiculescuS Schneider-Maunoury
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 5, 2003·Genes & Development·Martine JaegleDies Meijer
Mar 12, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Johanna BuchstallerNed Mantei
Dec 8, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Masanori Takahashi, Noriko Osumi
Sep 2, 2005·Nature Reviews. Neuroscience·Kristjan R Jessen, Rhona Mirsky
Jan 22, 2008·Molecular and Cellular Neurosciences·Satu PäiväläinenAnthony M Heape
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jun 16, 2009·Nature Neuroscience·Ashwin WoodhooKristján R Jessen
Feb 16, 2011·Glia·Peter Arthur-FarrajKristján R Jessen
May 23, 2014·Genesis : the Journal of Genetics and Development·Yaqi DengQ Richard Lu
Dec 3, 2014·Cold Spring Harbor Protocols·Amanda Brosius Lutz
Apr 30, 2015·Glia·Kelly R MonkCarla Taveggia
Jun 10, 2015·Cold Spring Harbor Perspectives in Biology·James L Salzer
Mar 17, 2018·Methods in Molecular Biology·Jihyun Kim, Haesun A Kim
Jul 15, 2018·Methods in Molecular Biology·Carla Taveggia, Alessandra Bolino
Apr 12, 2019·Frontiers in Molecular Neuroscience·Kristjan R Jessen, Rhona Mirsky
Aug 10, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Elias SabagYankel Gabet
Nov 14, 2019·Development·Robert FledrichRuth M Stassart

❮ Previous
Next ❯

Software Mentioned

Excel
Ensembl
Precision Melt Analysis
Prism
Primer
NormFinder
Graph Pad Prism
R

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.