RETRACTED: The Anaphase-Promoting Complex/Cyclosome Is Essential for Entry into Meiotic M-Phase

Developmental Cell
Saurav MalhotraThomas U Mayer

Abstract

Vertebrate immature oocytes are arrested at prophase of meiosis I (MI). Hormonal stimulation breaks this prophase-I arrest and induces re-entry into MI. The mechanism underlying meiotic resumption remains largely elusive. Here, we demonstrate that the anaphase-promoting complex/cyclosome (APC/C) in complex with Cdh1 has an unexpected function in meiosis in that it is essential for meiotic resumption. We identify the catalytic subunit of protein phosphatase 6 (PP6c) as the critical substrate whose APC/C(Cdh1)-mediated destruction is a prerequisite for the re-entry of immature Xenopus laevis oocytes into MI. Preventing PP6c destruction impairs activating autophosphorylation of Aurora A, a cell-cycle kinase critical for meiotic translation. Restoring meiotic translation rescues the meiotic resumption defect of Cdh1-depleted oocytes. Thus, our studies discover that the essential function of the APC/C in triggering cell-cycle transitions is not limited to M-phase exit but also applies to entry into meiotic M-phase, and identify a crucial APC/C-PP6c-Aurora A axis in the resumption of female meiosis.

References

May 4, 2000·Molecular Biology of the Cell·E R KramerJ M Peters
Jun 28, 2000·The Journal of Cell Biology·A D Rudner, A W Murray
Jun 11, 2002·Biochemical and Biophysical Research Communications·Yuan ZhouDong-Yan Jin
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brian C DuckworthJoan V Ruderman
Apr 18, 2003·Current Biology : CB·Patrick A EyersJames L Maller
Sep 6, 2003·Current Biology : CB·Hartmut C VodermaierJan-Michael Peters
Dec 6, 2003·The EMBO Journal·Claudine KraftJan-Michael Peters
Jul 10, 2004·Developmental Biology·Catherine PapinElisabeth Mandart
Feb 17, 2005·Genes & Development·Andreas SchmidtThomas U Mayer
Jan 19, 2006·Genes & Development·Kiran Padmanabhan, Joel D Richter
May 23, 2006·Nature Cell Biology·Alexandra ReisKeith T Jones
Jun 10, 2006·The EMBO Journal·Amanda CharlesworthAngus M MacNicol
Sep 25, 2007·Nature Cell Biology·Alexandra ReisKeith T Jones
Jan 12, 2008·Biochemistry·Bjarki StefanssonDavid L Brautigan
Jun 14, 2008·Molecular Biology of the Cell·Wanli TangSally Kornbluth
Jan 9, 2009·Biology of Reproduction·Karen Schindler, Richard M Schultz
Apr 7, 2012·EMBO Reports·Satoru Mochida, Tim Hunt
May 2, 2014·The EMBO Journal·Susanne HellmuthOlaf Stemmann

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Citations

Jan 10, 2017·PLoS Genetics·Encar García-OliverJérôme Govin
Jan 18, 2017·Genesis : the Journal of Genetics and Development·Amy K Sater, Sally A Moody
Sep 21, 2016·Molecular Reproduction and Development·Yoshie A FujiokaKunihiko Naito

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