PMID: 9416479Jan 7, 1998Paper

Retrieved endogenous biotin: a novel marker and a potential pitfall in diagnostic immunohistochemistry

Histopathology
G BussolatiM Papotti

Abstract

Antigen retrieval (AR) procedures are based on the effect of heating (by either microwave or pressure cooking treatments) on routinely fixed and paraffin embedded tissues. We observed that AR procedures restore the reactivity of endogenous biotin (EB) and report on the distribution of EB following AR in a series of routinely fixed and embedded tissues. Following pressure cooking or microwave treatments, a simple streptavidin-peroxidase staining revealed retrieved endogenous biotin (REB) in normal tissues (such as liver, kidney and adrenal cortex), in oxyphylic cells and in some tumours, especially in carcinomas of the kidney and of the adrenal cortex. In formalin-fixed (but not in alcohol-fixed) tissue sections, the heating procedures caused an intense and finely granular cytoplasmic reaction, following a routine streptavidin-conjugated peroxidase treatment. The staining was prevented by blocking of EB by a sequential avidin-biotin treatment. Retrieval of EB reactivity can cause pitfalls in diagnostic immunohistochemistry but, alternatively, it might also constitute a useful and novel diagnostic marker.

Citations

Jun 11, 2002·Experimental Parasitology·Vitorino Modesto dos SantosLuis Eduardo Ramírez
Jul 16, 2003·Veterinary Microbiology·Romeo E SanchezMaurice B Pensaert
Jan 10, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Josef RüschoffGiuseppe Viale
Sep 3, 1998·Histopathology·S J NaylerK Cooper
Oct 10, 2007·The Journal of Experimental Medicine·Yongxiong ChenChen-Lung Steve Lin
Mar 17, 2006·Applied Immunohistochemistry & Molecular Morphology : AIMM·Tine Plato HansenClaus Fenger
Sep 5, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Wei HuangThomas Pier
Dec 23, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Anitha KamathDhanpat Jain
Nov 14, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Lise LyckHenrik Daa Schrøder
Jul 21, 2005·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Daniel A DavisRobert A Robinson
Feb 24, 2006·Virchows Archiv : an International Journal of Pathology·Marco VolanteGianni Bussolati
Mar 15, 2002·Applied Immunohistochemistry & Molecular Morphology : AIMM·Merce JordaMehrdad Nadji
Sep 15, 2001·Applied Immunohistochemistry & Molecular Morphology : AIMM·M PapottiG Bussolati
Mar 16, 2000·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·M PapottiG Bussolati
Sep 2, 2008·Expert Review of Anticancer Therapy·Obi L GriffithSam M Wiseman
Jan 13, 2006·Histopathology·A Bartolazzi, G Bussolati
Dec 7, 2007·Journal of Cellular and Molecular Medicine·Razvan Tudor Radulescu, Thomas Boenisch
Oct 25, 2011·Diagnostic Cytopathology·Xin JingConstantine G A Theoharis
Apr 20, 1999·Human Pathology·F FogtC Poremba
Sep 18, 2014·Asia-Pacific Journal of Clinical Oncology·Kyoung-Mee KimSheng Zhang
Jan 25, 2011·Molecular and Cellular Endocrinology·Alex Paul, Ed Laufer
Apr 7, 2010·The American Journal of Pathology·Connie G ChiuSam M Wiseman
Jul 17, 2014·Renal Failure·Konstantinos KoutroutsosLydia Nakopoulou
Mar 30, 2001·Applied Immunohistochemistry & Molecular Morphology : AIMM·K HashizumeY Tani
Jul 18, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M VolanteG Bussolati
Jul 12, 2005·Veterinary Pathology·J A Ramos-Vara
Feb 24, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M VolanteG Bussolati
Aug 9, 2001·International Journal of Surgical Pathology·Laura VibertiGianni Bussolati
Jul 10, 2008·International Journal of Surgical Pathology·Rania BakkarHouda Alatassi
Nov 4, 2008·International Journal of Surgical Pathology·Prisca SozzaniChiara Benedetto
Aug 16, 2001·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·A SapinoG Bussolati
Sep 29, 2018·Current Protocols in Cell Biology·Lisa PrevedelEliseo A Eugenin
Mar 1, 2012·Virchows Archiv : an International Journal of Pathology·Valeria BarresiGaetano Barresi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved