PMID: 2509551Nov 1, 1989Paper

Retrieving vesicles in secretion-induced rat chromaffin cells contain fodrin

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
T Fujimoto, K Ogawa

Abstract

We examined the distribution of fodrin and cytochrome b561 in secretion-induced rat chromaffin cells (epinephrine cells) by immunofluorescence and immunoelectron microscopy. Fasted rats injected with a large dose of insulin were perfusion-fixed and frozen sections of the adrenal medulla were immunolabeled. Fodrin, a peripheral membrane protein, was distributed only in the cell periphery in control cells, but was observed in the cell interior after the insulin treatment; many of the markers were found around small vesicles, 50-200 nm in diameter, and large vacuoles, more than 500 nm in diameter. On the other hand, cytochrome b561, an integral membrane protein, was seen only in the chromaffin granules in control cells, and appeared in small vesicles in the stimulated cells but not in large vacuoles. By double immunolabeling it was shown that cytochrome b561 coexisted with fodrin in the small vesicles. The coexistence of the two proteins was confirmed by the labeling of subcellular particles immunoadsorbed from the insulin-treated adrenal medulla homogenate; vesicles immunoisolated with anti-fodrin antibody on polyacrylamide beads were positively immunolabeled with anti-cytochrome b561 antibody. The present results show that durin...Continue Reading

Citations

Oct 1, 1992·Seminars in Cell Biology·S A Tooze, J C Stinchcombe
Feb 12, 1998·The American Journal of Physiology·T L HerringC E Morris
Aug 1, 1997·Experimental Cell Research·T AokiT Fujimoto

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