Retrocyclin 2: a new therapy against avian influenza H5N1 virus in vivo and vitro

Biotechnology Letters
Qing-long LiangHong-xuan He

Abstract

We tested the ability of retrocyclin 2, a type of theta defensin, to protect cells and chicken embryos from infection by H5N1 highly pathogenic avian influenza virus. A gene fragment of retrocyclin 2 was designed based on the protein sequence of retrocyclin 2 and cloned into the eukaryotic expression vector pcDNA4.01 (HismaxA), named pcDNA4-RC2. The expression vector pcDNA4-RC2 protected MDCK cells and chicken embryos from infection by the H5N1 virus through inhibition of virus replication and viral mRNA transcription. Retrocyclin 2 is therefore effective in preventing H5N1 virus infection in vivo and in vitro and could be considered as a new therapy for H5N1 influenza and other diseases.

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Citations

May 8, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Emily Gwyer FindlayDonald J Davidson
Jun 16, 2012·The Journal of Biological Chemistry·Robert I LehrerMichael E Selsted
Mar 8, 2013·Future Medicinal Chemistry·Derry K Mercer, Deborah A O'Neil
Jun 27, 2013·Medicinal Research Reviews·Evelien Vanderlinden, Lieve Naesens
Mar 31, 2015·Journal of Virological Methods·Lixiang JiangHongxuan He
Nov 13, 2013·Innate Immunity·Shweta TripathiKevan L Hartshorn
Apr 27, 2010·Innate Immunity·Tesfaldet TecleKevan L Hartshorn
Feb 22, 2012·Antiviral Chemistry & Chemotherapy·Yan JiangMingyuan Li
Aug 1, 2014·Angewandte Chemie·Anne C Conibear, David J Craik
Feb 22, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mona DossKevan L Hartshorn

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