Retrograde axonal tracing with fluorescent markers.

Current Protocols in Neuroscience
B R Schofield

Abstract

The growth of fluorescence imaging technology and the development of sensitive fluorescent retrograde tracers has provided many new approaches for analyzing neuronal circuits. Fluorescent markers provide unparalleled opportunity for combining axonal tract tracing with techniques such as immunohistochemistry or physiological recording. This unit describes the use of six different fluorescent tracers: Fast Blue, fluorescein dextran, FluoroGold, FluoroRuby, red beads, and green beads. Guidance is provided on how to choose a tracer for a particular experiment, and three methods are described for injecting the tracers, including pressure injection through a microsyringe or a micropipet, and iontophoretic injection through a micropipet. Criteria for selecting the most appropriate method are discussed. The protocols provide the information necessary to take advantage of the numerous fluorescent tracers that are available and to apply them to a wide variety of scientific questions.

References

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Mar 25, 2006·Journal of the Association for Research in Otolaryngology : JARO·Brett R SchofieldRyan M Schofield

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Citations

Jun 25, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mani Vessal, Corinna Darian-Smith
Feb 24, 2016·Frontiers in Neuroanatomy·Gay R HolsteinGiorgio P Martinelli
Apr 20, 2014·Frontiers in Neuroanatomy·Jeffrey G MellottBrett R Schofield
Mar 19, 2014·Frontiers in Neuroanatomy·Brett R SchofieldNichole L Foster
Nov 22, 2014·Frontiers in Neuroanatomy·Jeffrey G MellottBrett R Schofield
Aug 8, 2014·Frontiers in Neuroanatomy·Brett R SchofieldSusan D Motts
Jul 16, 2013·Nature Neuroscience·Sabrina D RobertsonPatricia Jensen
Jun 6, 2019·Journal of Neurophysiology·Amelia H GagliusoGay R Holstein
Dec 11, 2013·The Journal of Comparative Neurology·Gay R HolsteinGiorgio P Martinelli
Dec 31, 2015·Journal of Neuroendocrinology·W A ZuureG M Anderson
Jul 15, 2016·Experimental Brain Research·Gay R HolsteinGiorgio P Martinelli
Feb 25, 2017·Innovative Surgical Sciences·Christine RadtkePeter M Vogt
Jan 6, 2018·Brain Structure & Function·Jeffrey G MellottBrett R Schofield
Apr 1, 2017·Science·Kevin YackleMark A Krasnow
Sep 12, 2019·Frontiers in Neuroscience·Christine SaleebaSimon McMullan
Nov 19, 2019·Frontiers in Systems Neuroscience·Demetrio MilardiAlberto Cacciola
Sep 28, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jordan M Blacktop, Barbara A Sorg
Jan 30, 2021·Brain Structure & Function·Gabriela B Gómez-González, Ataúlfo Martínez-Torres
Jul 28, 2012·Experimental Neurology·Claudia J Laskowski, Frank Bradke
Aug 3, 2021·Frontiers in Neural Circuits·Nichole L BeebeBrett R Schofield

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