Retrograde axonal transport of locally synthesized proteins, e.g., actin and heat shock protein 70, in regenerating adult frog sciatic sensory axons

Journal of Neuroscience Research
M EdbladhA Edström

Abstract

The local synthesis and subsequent retrograde axonal transport of [35S]methionine-labelled proteins was studied in the in vitro regenerating adult frog sciatic sensory axons. By the use of a three compartment culture system, proteins in the outgrowth region were selectively labelled. After 2 days in culture a rise in TCA-insoluble radioactivity was detected in the dorsal root ganglia, which could be prevented by the addition of vinblastine or 2,4-dinitrophenol to the nerve proximal to the crush site. Two-dimensional polyacrylamide gel electrophoresis of ganglionic proteins revealed a pattern of 35 labelled polypeptides with apparent molecular masses (Mm) ranging from < 15 to 95 kDa and with isoelectric points (pI) ranging from 4.5 to 6.5. The major ones, representing about 75% of the activity in a gel, were of Mm/pI 47/5.4, 48/6.1,. 57/6.0, 62/5.2, 65/4.9-5.0, 65/5.2, and 81/5.4 respectively. One of these polypeptides (47/5.4) was identified as actin and another (81/5.4) as a member of the heat shock protein 70 family. The spots at 65/4.9-5.0 were tubulin isoforms. There was a striking similarity between transported proteins on one hand, and proteins synthesized in the injured nerve on the other, with respect to the Mm/pI of at...Continue Reading

References

Aug 1, 1977·Journal of Neurochemistry·M A Bisby, V T Bulger
Apr 1, 1992·Journal of Neurobiology·T E Buchheit, M Tytell
Sep 18, 1992·Brain Research. Developmental Brain Research·B Tedeschi, F J Liuzzi
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S RotshenkerE M Shooter
Apr 1, 1990·Journal of Neuroscience Methods·M EdbladhA Edström
Jun 1, 1990·Journal of Neurochemistry·M J SavageD J Goldberg
Aug 1, 1986·The Journal of Cell Biology·M J Schlesinger
Oct 1, 1986·Journal of Neurocytology·P M Richardson, V M Verge
Jan 15, 1986·Brain Research·M TytellR J Lasek
Oct 26, 1973·Brain Research·A Edström, M Hanson
Mar 15, 1974·Brain Research·I A HendryL L Iversen
Aug 23, 1974·Brain Research·K StöckelH Thoenen
Oct 1, 1980·Physiological Reviews·H Thoenen, Y A Barde
Jan 1, 1980·Journal of Neurochemistry·G P Harper, H Thoenen
Dec 24, 1984·Brain Research·M Tytell, R J Lasek
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J H Skene, E M Shooter
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K W MintonA P Minton
Dec 1, 1981·The Journal of Cell Biology·D J Carey, R P Bunge

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Citations

Dec 5, 1998·Brain Research. Molecular Brain Research·A K MurashovD J Wolgemuth
Mar 16, 2011·Biochemistry Research International·Giuseppina TurturiciFabiana Geraci
Jul 24, 2004·Cell Stress & Chaperones·J Lille TidwellMichael Tytell
Feb 9, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·John J Heikkila
Apr 11, 2012·Journal of Neuroscience Research·Xinjing LuoHaixiao Chen
Jul 29, 2005·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M Tytell
Jun 12, 1998·The Journal of Comparative Neurology·R A ShellerG D Bittner
Jan 6, 2004·Journal of Neurobiology·M W Weible, I A Hendry
Aug 19, 2007·Developmental Neurobiology·Anna R TaylorCarolanne E Milligan
Mar 9, 2017·Frontiers in Neuroscience·Shalina S OusmanErin-Mai F Lim

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