Retrograde axonal transport of rabies virus is unaffected by interferon treatment but blocked by emetine locally in axons

PLoS Pathogens
Margaret A MacGibenyLynn W Enquist

Abstract

Neuroinvasive viruses, such as alpha herpesviruses (αHV) and rabies virus (RABV), initially infect peripheral tissues, followed by invasion of the innervating axon termini. Virus particles must undergo long distance retrograde axonal transport to reach the neuron cell bodies in the peripheral or central nervous system (PNS/CNS). How virus particles hijack the axonal transport machinery and how PNS axons respond to and regulate infection are questions of significant interest. To track individual virus particles, we constructed a recombinant RABV expressing a P-mCherry fusion protein, derived from the virulent CVS-N2c strain. We studied retrograde RABV transport in the presence or absence of interferons (IFN) or protein synthesis inhibitors, both of which were reported previously to restrict axonal transport of αHV particles. Using neurons from rodent superior cervical ganglia grown in tri-chambers, we showed that axonal exposure to type I or type II IFN did not alter retrograde axonal transport of RABV. However, exposure of axons to emetine, a translation elongation inhibitor, blocked axonal RABV transport by a mechanism that was not dependent on protein synthesis inhibition. The minority of RABV particles that still moved retro...Continue Reading

References

Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B DietzscholdH Koprowski
Oct 1, 1981·The Journal of General Virology·H D WatsonA L Smith
Jan 1, 1997·The Journal of General Virology·H RauxD Blondel
Jul 15, 1999·Journal of Neurocytology·P Lewis, T L Lentz
Jan 6, 2000·Nature Cell Biology·S J King, T A Schroer
Oct 12, 2000·Journal of Virology·H RauxD Blondel
Oct 12, 2000·Journal of Virology·Y JacobN Tordo
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Milosz FaberBernhard Dietzschold
Jan 14, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Poonam VermaJames W Fawcett
Apr 5, 2005·Journal of Neurovirology·Monique Lafon
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gene S TanMatthias J Schnell
Oct 12, 2007·Journal of Virology·Yvonne KlingenStefan Finke
Jan 15, 2008·Nature Cell Biology·Llewellyn J CoxSamie R Jaffrey
Nov 12, 2010·Journal of Virology·Christoph Wirblich, Matthias J Schnell
Nov 2, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David J MitchellK Kevin Pfister
Feb 19, 2013·Cell Host & Microbe·Sofia V ZaichickGregory A Smith
Apr 23, 2014·The Journal of Biological Chemistry·James N HislopNicholas D Mazarakis
Nov 7, 2014·Neuron·Sandra MadayErika L F Holzbaur
Mar 10, 2016·Annual Review of Virology·Benjamin M DavisMatthias J Schnell
Jun 24, 2016·PLoS Pathogens·Rupkatha MukhopadhyayRavit Arav-Boger
Dec 1, 2017·BMC Bioinformatics·Curtis T RuedenKevin W Eliceiri

❮ Previous
Next ❯

Citations

Feb 29, 2020·PLoS Pathogens·Pavithra AravamudhanTerence S Dermody
Mar 29, 2020·Viruses·Vinod SundaramoorthyMegan Dearnley
Jun 10, 2020·Genes, Brain, and Behavior·Richard Lathe, David St Clair
Aug 8, 2020·Frontiers in Microbiology·Yimin WangYuan Sun
Aug 28, 2020·Frontiers in Cellular Neuroscience·Matija FenrichMarija Heffer
Jun 18, 2020·Viruses·Aleksandr IanevskiDenis E Kainov
Jul 29, 2020·Comptes rendus biologies·Jean-Pierre ChangeuxMakoto Miyara
Feb 23, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Petter I AndersenDenis E Kainov
Sep 2, 2021·Journal of Virology·Daniel Gavino-LeopoldinoIranaia Assunção-Miranda

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
Assay
PCR
restriction digest
fluorescence
transfection

Software Mentioned

NIS Elements Advanced Research
GraphPad
Graphpad Prism
Fiji KymographBuilder
Fiji
Fiji Z
Fiji Image J

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.