Retrograde interferon-gamma signaling induces major histocompatibility class I expression in human-induced pluripotent stem cell-derived neurons

Annals of Clinical and Translational Neurology
Benjamin D S ClarksonCharles L Howe

Abstract

Injury-associated axon-intrinsic signals are thought to underlie pathogenesis and progression in many neuroinflammatory and neurodegenerative diseases, including multiple sclerosis (MS). Retrograde interferon gamma (IFNγ) signals are known to induce expression of major histocompatibility class I (MHC I) genes in murine axons, thereby increasing the susceptibility of these axons to attack by antigen-specific CD8+T cells. We sought to determine whether the same is true in human neurons. A novel microisolation chamber design was used to physically isolate and manipulate axons from human skin fibroblast-derived induced pluripotent stem cell (iPSC)-derived neuron-enriched neural aggregates. Fluorescent retrobeads were used to assess the fraction of neurons with projections to the distal chamber. Axons were treated with IFNγfor 72 h and expression of MHC class I and antigen presentation genes were evaluated by RT-PCR and immunofluorescence. Human iPSC-derived neural stem cells maintained as 3D aggregate cultures in the cell body chamber of polymer microisolation chambers extended dense axonal projections into the fluidically isolated distal chamber. Treatment of these axons with IFNγresulted in upregulation of MHC class I and antigen...Continue Reading

References

Oct 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Oleg N SuslovDennis A Steindler
Jan 23, 2003·Brain : a Journal of Neurology·M FilippiA Falini
Dec 4, 2003·Brain Research·Alessandra BezEugenio Agostino Parati
Jan 6, 2004·Journal of Neurobiology·Christopher S von Bartheld
May 19, 2005·Journal of the Neurological Sciences·Peter K Stys
Aug 10, 2005·PLoS Biology·Luciano ContiAustin Smith
Aug 12, 2005·Nature Methods·Anne M TaylorNoo Li Jeon
Sep 10, 2005·Molecular and Cellular Neurosciences·Ondrej MachonZbynek Kozmik
Aug 19, 2006·Science·Josh SykenCarla J Shatz
Nov 23, 2006·Neurobiology of Disease·Charles L HoweMoses Rodriguez
Mar 16, 2007·Journal of Neurology·Massimiliano CalabresePaolo Gallo
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·C Alex GoddardCarla J Shatz
Sep 4, 2007·Brain Research Reviews·Sebastian ThamsStaffan Cullheim
Nov 15, 2007·Synapse·Mario SabhaAlexandre Leite Rodrigues De Oliveira
Dec 7, 2007·The Journal of Clinical Investigation·Clare L ParishErnest Arenas
Jul 18, 2008·Multiple Sclerosis : Clinical and Laboratory Research·A Wilkins, N Scolding
Feb 11, 2009·Cold Spring Harbor Symposia on Quantitative Biology·Y Elkabetz, L Studer
Feb 24, 2009·Lancet Neurology·Bruce D Trapp, Peter K Stys
Mar 20, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Diego CentonzeGianvito Martino
Jun 13, 2009·Experimental Neurology·Laura F GumyJames W Fawcett
Jun 23, 2009·Neurobiology of Aging·Wayne W PoonCarl W Cotman
Jun 26, 2009·Biomedical Microdevices·Jaewon ParkArum Han
Aug 15, 2009·Journal of Neuropathology and Experimental Neurology·Chandra DebCharles L Howe
Mar 9, 2010·Trends in Molecular Medicine·David P Stirling, Peter K Stys
May 4, 2010·Trends in Neurosciences·Eran PerlsonErika L F Holzbaur
Jul 17, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·G MandolesiDiego Centonze
Oct 1, 2010·Advanced Materials·Matthias P Lutolf, Helen M Blau
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lawrence FourgeaudLisa M Boulanger
Mar 2, 2011·Nature Neuroscience·Marian W GlynnA Kimberley McAllister
Apr 17, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tanja ScheiklRoland S Liblau
May 10, 2012·Experimental Neurology·Shigeki Tsutsui, Peter K Stys
Aug 9, 2012·Progress in Retinal and Eye Research·David J Calkins
Sep 4, 2012·Trends in Neurosciences·Bradford M Elmer, A Kimberley McAllister
Oct 4, 2012·Trends in Neurosciences·Larry J Millet, Martha U Gillette

❮ Previous
Next ❯

Citations

Dec 14, 2018·Nature Reviews. Neuroscience·Carrie E MahoneyThomas E Scammell
Aug 19, 2021·Cancer Immunology, Immunotherapy : CII·Haruka MiyataToshihiko Torigoe

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
PCR

Software Mentioned

Excel
ImageJ
Zen
NIH

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.