PMID: 8958794Nov 1, 1996Paper

Retrogradely labelled neurosecretory neurones of the rat hypothalamic arcuate nucleus express Fos protein following systemic injection of GH-releasing peptide-6

The Journal of Endocrinology
S L DicksonG Leng

Abstract

Previously, we demonstrated that the synthetic hexapeptide GH-releasing peptide (GHRP-6) activates a subpopulation of arcuate neurones, as reflected by increased electrical activation and by the detection of Fos protein in cell nuclei. Here we set out to determine (1) what proportion of the cells activated by GHRP-6 are neurosecretory neurones and (2) whether the cells activated by GHRP-6 contain tyrosine hydroxylase (TH; a marker of dopaminergic cells in this region) or beta-endorphin. In the first study, adult male rats were injected i.v. with the retrograde tracer, Fluorogold, to detect cells which project outside the blood-brain barrier (and are therefore likely to be neurosecretory neurones). Three days later the conscious rats were injected i.v. with 50 micrograms GHRP-6 and the brains processed for the immunocytochemical detection of Fos protein. Between 68% and 82% of the arcuate neurones expressing Fos protein following GHRP-6 injection were retrogradely labelled with Fluorogold. In the second study, conscious male rats, bearing a chronically implanted jugular catheter, were killed 90 min following an i.v. injection of 50 micrograms GHRP-6 and the brains were processed for the double immunocytochemical detection of Fos...Continue Reading

Citations

May 15, 2002·European Journal of Pharmacology·Giampiero MuccioliEzio Ghigo
Oct 26, 1999·Drug Discovery Today·M AnkersenA M Kappelgaard
Apr 20, 2002·Journal of Neuroendocrinology·G E Hoffman, D Lyo
Apr 9, 2013·Indian Journal of Endocrinology and Metabolism·Alfonso Abizaid, Tamas L Horvath
Feb 3, 2005·Nutritional Neuroscience·C GotteroE Ghigo
Aug 3, 1999·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·S L DicksonG Leng
Jun 20, 2003·Journal of Neuroscience Research·Odile ViltartHenrique Sequeira
Apr 9, 2005·Endocrine Reviews·Roy G Smith
Aug 11, 2007·Endocrinology·Miro FaouziHeike Münzberg
Jun 8, 2004·Endocrine Reviews·Aart J van der LelyEzio Ghigo
Mar 20, 2013·Journal of Pediatric Endocrinology & Metabolism : JPEM·Anastasia IbbaSandro Loche

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