Retrospective detection of exposure to sulfur mustard: improvements on an assay for liquid chromatography-tandem mass spectrometry analysis of albumin-sulfur mustard adducts

Journal of Analytical Toxicology
D NoortJohn R Barr

Abstract

We here report on the further development of the method comprising the pronase digestion of albumin alkylated by sulfur mustard and the subsequent mass spectrometric analysis of an adducted tripeptide. This includes significant improvements in both the albumin isolation procedure and the automation of the microliquid chromatography-electrospray-tandem mass spectrometric analysis. We also report on the results of a small reference range study, in which we have established that there are no detectable interferences in sera from unexposed individuals.

Citations

Mar 9, 2007·Archives of Toxicology·Nichola H WilliamsRobin M Black
Jun 21, 2007·Journal of Analytical Toxicology·Michael L SmithMarilyn A Huestis
Jan 27, 2005·Journal of Mass Spectrometry : JMS
Nov 24, 2018·Drug Testing and Analysis·Markus SiegertHarald John
May 9, 2015·The Journal of Biological Chemistry·Khyatiben V PathakRobert J Turesky
May 19, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Micaela C DamstenNico P E Vermeulen
Jan 11, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Florine HallezValérie Pichon

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