Retroviral gene transduction into chicken embryo gonads through blood circulation

Journal of Bioscience and Bioengineering
Yoshinori KawabeMasamichi Kamihira

Abstract

A retroviral vector was injected into the heart of developing chicken embryos to determine the best timing for effective gene transduction into the gonads. Transduction efficiency and transgene expression were both highest when the viral solution was injected into embryos at stages 14-15 (Hamburger-Hamilton stages), in which primordial germ cells (PGCs) can be found in the blood circulation. This study shows that PGCs in the blood are important targets for gene transfer into the gonads using retroviral vectors.

References

Dec 10, 1999·The Journal of Experimental Zoology·A TajimaT Chikamune
Aug 21, 2001·Biochemical and Biophysical Research Communications·S MizuaraiS Iijima
May 26, 2004·Trends in Biotechnology·Wilfried A Kues, Heiner Niemann
Aug 7, 2004·Mechanisms of Development·Helen Sang
Nov 23, 2006·Journal of Bioscience and Bioengineering·Yoshinori KawabeShinji Iijima
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·James N Petitte, Paul E Mozdziak
Jan 19, 2008·Biochemical and Biophysical Research Communications·Daisuke KodamaShinji Iijima
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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Citations

Apr 21, 2012·Animal Science Journal = Nihon Chikusan Gakkaihō·Peng SunZandong Li
Oct 21, 2021·Biology of Reproduction·Nima DehdilaniHesam Dehghani

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