Retroviral recombination is nonrandom and sequence dependent

Virology
D P WooleyR A Smith

Abstract

Sequence variation plays a significant role in the pathogenesis and persistence of retroviral infections and is a major obstacle in the development of vaccines as well as therapies against lethal diseases caused by retroviruses. Recombination is one means by which sequence variation is generated. However, the basic molecular mechanisms of recombination are not adequately understood. In the present study, a spleen necrosis virus (SNV) recombination system was used to ask whether a known hot spot for mutation was also a hot spot for retroviral recombination. The system consisted of a pair of SNV vectors expressing two drug-resistance genes, constructed so that recombinants could be selected by a double resistant phenotype. Restriction enzyme site differences engineered into the vectors were used to map the location of recombination sites within relatively small intervals (55 to 420 bp). The vectors were modified to create two pairs that differed only by the presence of runs of identical nucleotides. The runs of identical nucleotides had been shown previously to be hot spots for frameshift mutations during SNV reverse transcription. Each vector pair was introduced into DSDh helper cells by infection. Viruses were harvested from do...Continue Reading

References

Jun 11, 1976·Science·H M Temin
Jul 1, 1992·Journal of Virology·W S Hu, H M Temin
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A MorrisonA Sugino
Nov 30, 1990·Science·W S Hu, H M Temin
Sep 4, 1990·Biochemistry·T A Kunkel
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W S Hu, H M Temin
Nov 25, 1988·Science·B D PrestonL A Loeb
Nov 25, 1988·Science·J D RobertsT A Kunkel
Dec 1, 1986·Molecular and Cellular Biology·J P Dougherty, H M Temin
Mar 15, 1968·International Journal of Cancer. Journal International Du Cancer·H M Temin
Oct 1, 1974·The Journal of General Virology·J L RiggsE H Lennette
Jun 1, 1984·Molecular and Cellular Biology·S Kawai, M Nishizawa
Jan 1, 1994·Journal of Virology·J S JonesH M Temin

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Citations

Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Negroni, H Buc
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Adewunmi Onafuwa-Nuga, Alice Telesnitsky
Feb 24, 2015·Retrovirology·Paweł M BęczkowskiMargaret J Hosie
Nov 9, 2001·Annual Review of Genetics·M Negroni, H Buc
Dec 31, 2002·Virology·María José Iglesias-Sánchez, Cecilio López-Galíndez
Dec 29, 2013·Journal of Virology·Redmond P SmythMiles P Davenport
Jul 4, 2001·The Journal of Biological Chemistry·M BalakrishnanR A Bambara

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