PMID: 9445018Jan 28, 1998Paper

Retroviral recombination rates do not increase linearly with marker distance and are limited by the size of the recombining subpopulation

Journal of Virology
J A AndersonW S Hu

Abstract

Recombination occurs at high frequencies in all examined retroviruses. The previously determined homologous recombination rate in one retroviral replication cycle is 4% for markers 1.0 kb apart in spleen necrosis virus (SNV). This has often been used to suggest that approximately 30 to 40% of the replication-competent viruses with 7- to 10-kb genomes undergo recombination. These estimates were based on the untested assumption that a linear relationship exists between recombination rates and marker distances. To delineate this relationship, we constructed three sets of murine leukemia virus (MLV)-based vectors containing the neomycin phosphotransferase gene (neo) and the hygromycin phosphotransferase B gene (hygro). Each set contained one vector with a functional neo and an inactivated hygro and one vector with a functional hygro and an inactivated neo. The two inactivating mutations in the three sets of vectors were separated by 1.0, 1.9, and 7.1 kb. Recombination rates after one round of replication were 4.7, 7.4, and 8.2% with markers 1.0, 1.9, and 7.1 kb apart, respectively. Thus, the rate of homologous recombination with 1.0 kb of marker distance is similar in MLV and SNV. The recombination rate increases when the marker di...Continue Reading

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Citations

Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·C K HwangV K Pathak
Nov 9, 2001·Annual Review of Genetics·M Negroni, H Buc
Sep 28, 2006·Nucleic Acids Research·Heather A BairdMatteo Negroni
Apr 15, 2009·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·Kazushi Motomura
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Adewunmi Onafuwa-Nuga, Alice Telesnitsky
Apr 8, 2000·Journal of Biomedical Science·J G Mikkelsen, F S Pedersen
Nov 18, 2005·The Journal of Biological Chemistry·Román GalettoMatteo Negroni
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mario P S ChinWei-Shau Hu
Dec 24, 2013·The Journal of General Virology·Nicolas TromasSantiago F Elena
Feb 24, 2015·Retrovirology·Paweł M BęczkowskiMargaret J Hosie
Sep 26, 2014·Viruses·Jonathan M O Rawson, Louis M Mansky
Jun 25, 2015·Microbiology Spectrum·Stephen H Hughes
Mar 6, 2007·Virology·Mario P S ChinWei-Shau Hu
Apr 8, 2009·Retrovirology·Kathleen GärtnerCarsten Scheller
Mar 1, 2008·Virus Research·Bertha Cecilia RamirezMatteo Negroni

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