REV1 promotes PCNA monoubiquitylation through interacting with ubiquitylated RAD18

Journal of Cell Science
Zhifeng WangCaixia Guo

Abstract

Translesion DNA synthesis (TLS) is a mode of DNA damage tolerance which plays an important role in genome mutagenesis and chromatin integrity maintenance. Proliferating cell nuclear antigen (PCNA) monoubiquitylation is one of the key factors for TLS pathway choice. So far, it remains unclear how the TLS pathway is elaborately regulated. Here, we report that TLS polymerase REV1 can promote PCNA monoubiquitylation after UV radiation. Further studies revealed that this stimulatory effect is mediated through the enhanced interaction between REV1 and ubiquitylated RAD18, which facilitates the release of nonubiquitylated RAD18 from ubiquitylated RAD18 trapping, after which RAD18 is recruited to chromatin for its TLS function. Furthermore, we found that this stimulatory effect could also be detected after exposure to hydroxyurea or mitomycin C, but not methyl methanesulfonate (MMS), which is in line with the fact that ubiquitylated RAD18 could not be detected after exposure to MMS.

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Citations

Jul 6, 2016·Frontiers in Genetics·Lina CipollaSimone Sabbioneda
Mar 11, 2017·Critical Reviews in Biochemistry and Molecular Biology·Alexandra Vaisman, Roger Woodgate
Nov 16, 2017·Nucleic Acids Research·Juanjuan GongTie-Shan Tang
Feb 1, 2019·The Journal of Biological Chemistry·Mark HedglinStephen J Benkovic
Feb 21, 2019·Nucleic Acids Research·Zhifeng WangXingzhi Xu
Nov 19, 2019·Critical Reviews in Biochemistry and Molecular Biology·Yuji Masuda, Chikahide Masutani
Jan 27, 2020·Environmental and Molecular Mutagenesis·Xiaolu MaCaixia Guo
Apr 22, 2017·Oncotarget·Xuefei ZhuTie-Shan Tang
Jul 30, 2020·The Biochemical Journal·Li FanWei Xiao
Dec 28, 2018·Genes·Wendy LeungAnja-Katrin Bielinsky
Mar 29, 2018·Nucleic Acids Research·Min HuangCaixia Guo
Jun 30, 2019·Nucleic Acids Research·Bas PilzeckerHeinz Jacobs

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