REV3L, a promising target in regulating the chemosensitivity of cervical cancer cells

PloS One
Li YangXi Cheng

Abstract

REV3L, the catalytic subunit of DNA Polymerase ζ (Polζ), plays a significant role in the DNA damage tolerance mechanism of translesion synthesis (TLS). The role of REV3L in chemosensitivity of cervical cancer needs exploration. In the present study, we evaluated the expression of the Polζ protein in paraffin-embedded tissues using immunohistochemistry and found that the expression of Polζ in cervical cancer tissues was higher than that in normal tissues. We then established some cervical cancer cell lines with REV3L suppression or overexpression. Depletion of REV3L suppresses cell proliferation and colony formation of cervical cancer cells through G1 arrest, and REV3L promotes cell proliferation and colony formation of cervical cancer cells by promoting G1 phase to S phase transition. The suppression of REV3L expression enhanced the sensitivity of cervical cancer cells to cisplatin, and the overexpression of REV3L conferred resistance to cisplatin as evidenced by the alteration of apoptosis rates, and significantly expression level changes of anti-apoptotic proteins B-cell lymphoma 2 (Bcl-2), myeloid cell leukemia sequence 1 (Mcl-1) and B-cell lymphoma-extra large (Bcl-xl) and proapoptotic Bcl-2-associated x protein (Bax). Our ...Continue Reading

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Citations

Dec 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Haoran LiJiawei Chen
Dec 14, 2017·Journal of Cellular Biochemistry·Xiaoguang ChenJun Ma
Sep 10, 2019·World Journal of Gastroenterology : WJG·Wei-Bo DuWen-Biao Chen
Jun 14, 2019·Cell Death & Disease·Luciana Rodrigues GomesCarlos Frederico Martins Menck
Nov 13, 2020·Expert Opinion on Investigational Drugs·Seema M PatelM Kyle Hadden
Oct 14, 2020·Biochemistry. Biokhimii︠a︡·E S ShilkinA V Makarova
Dec 31, 2020·Cancers·Sebastian O WendelNicholas A Wallace

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Methods Mentioned

BETA
transfection
electrophoresis
PCR
Assay
flow cytometry
Reverse transcription PCR

Software Mentioned

Primer Premier
BLAST
AutoDimer
Image J
SPSS

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