Revealing early steps of alpha2beta1 integrin-mediated adhesion to collagen type I by using single-cell force spectroscopy

Molecular Biology of the Cell
Anna TaubenbergerClemens M Franz

Abstract

We have characterized early steps of alpha(2)beta(1) integrin-mediated cell adhesion to a collagen type I matrix by using single-cell force spectroscopy. In agreement with the role of alpha(2)beta(1) as a collagen type I receptor, alpha(2)beta(1)-expressing Chinese hamster ovary (CHO)-A2 cells spread rapidly on the matrix, whereas alpha(2)beta(1)-negative CHO wild-type cells adhered poorly. Probing CHO-A2 cell detachment forces over a contact time range of 600 s revealed a nonlinear adhesion response. During the first 60 s, cell adhesion increased slowly, and forces associated with the smallest rupture events were consistent with the breakage of individual integrin-collagen bonds. Above 60 s, a fraction of cells rapidly switched into an activated adhesion state marked by up to 10-fold increased detachment forces. Elevated overall cell adhesion coincided with a rise of the smallest rupture forces above the value required to break a single-integrin-collagen bond, suggesting a change from single to cooperative receptor binding. Transition into the activated adhesion mode and the increase of the smallest rupture forces were both blocked by inhibitors of actomyosin contractility. We therefore propose a two-step mechanism for the est...Continue Reading

References

Apr 15, 1994·Science·E L FlorinH E Gaub
May 15, 1996·The Biochemical Journal·K E KadlerJ A Chapman
Jun 1, 1996·The Journal of Cell Biology·M Chrzanowska-Wodnicka, K Burridge
Apr 1, 1997·Biophysical Journal·E Evans, K Ritchie
Sep 5, 1998·Trends in Cell Biology·P E Hughes, M Pfaff
Jun 22, 1999·Current Biology : CB·K RottnerJ V Small
Oct 8, 1999·Biophysical Journal·D Raucher, M P Sheetz
Sep 15, 2000·The Journal of Biological Chemistry·Y XuM Höök
Sep 27, 2001·The Journal of Biological Chemistry·M TullaJ Heino
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·B GeigerK M Yamada
Jun 18, 2002·Journal of Structural Biology·David J S Hulmes
Dec 24, 2002·The Journal of Biological Chemistry·Wan-Ming ZhangDonald Gullberg
Jan 28, 2003·Biophysical Journal·Feiya LiVincent T Moy
May 3, 2003·Current Opinion in Structural Biology·John W WeiselRustem I Litvinov
Oct 2, 2003·Current Opinion in Cell Biology·Christopher V Carman, Timothy A Springer
Jan 23, 2004·Biological Procedures Online·Ewa P. WojcikiewiczVincent T. Moy
May 18, 2004·The Journal of Biological Chemistry·Johanna JokinenJyrki Heino
May 19, 2004·The International Journal of Biochemistry & Cell Biology·Daniel J WhiteJyrki Heino
Jun 11, 2004·Annual Review of Biochemistry·Carlos BustamanteDavid Izhaky
Jul 17, 2004·European Biophysics Journal : EBJ·Harald JanovjakDaniel J Müller
Oct 1, 2004·Chembiochem : a European Journal of Chemical Biology·Miriam CohenLia Addadi
Nov 19, 2004·Microscopy Research and Technique·Fengzhi JiangDaniel J Müller
Aug 16, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Zhe SunGerald A Meininger
Sep 13, 2005·Journal of Cell Science·Pierre-Henri PuechCarl-Philipp Heisenberg
Dec 24, 2005·Journal of Cell Science·Porntula PanorchanDenis Wirtz
Feb 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Timothy V RattoMichael W McElfresh
Apr 8, 2006·Journal of Structural Biology·David A CisnerosDaniel J Muller
May 6, 2006·Ultramicroscopy·Pierre-Henri PuechDaniel J Muller
Jul 11, 2006·Journal of Biomechanics·Nathan D Gallant, Andrés J García

❮ Previous
Next ❯

Citations

Dec 7, 2007·Pflügers Archiv : European journal of physiology·Thomas LudwigUlrich S Schwarz
Jul 20, 2010·Biotechnology Letters·Katty Goossens, Ronnie Willaert
Oct 5, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ivan S YermolenkoTatiana P Ugarova
Dec 30, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·David LepzelterMuhammad Zaman
May 19, 2009·Nature Chemical Biology·Daniel J MüllerYves F Dufrêne
Jul 26, 2008·Nature Nanotechnology·Daniel J Müller, Yves F Dufrêne
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Aurélien DumètrePierre-Henri Puech
May 22, 2013·Tissue Engineering. Part B, Reviews·Anna V TaubenbergerDaniel J Muller
Dec 14, 2011·Applied and Environmental Microbiology·Aurélien DumètreIsabelle Villena
Apr 30, 2009·Annual Review of Biomedical Engineering·Deok-Ho KimYu Sun
Dec 17, 2009·PLoS Computational Biology·Matthew J PaszekDaniel A Hammer
Jul 30, 2011·PloS One·Pierre-Henri PuechPierre Bongrand
Apr 23, 2010·Biointerphases·Gilles WederMartha Liley
Mar 8, 2014·Micron : the International Research and Review Journal for Microscopy·Congzhou Wang, Vamsi K Yadavalli
May 27, 2014·Trends in Biotechnology·Orane Guillaume-GentilJulia A Vorholt
Mar 1, 2008·Cellular and Molecular Bioengineering·Elodie CretelPierre Bongrand
Sep 23, 2008·FEBS Letters·Mira TullaJyrki Heino
Aug 8, 2015·International Journal of Molecular Sciences·Amelia Ahmad Khalili, Mohd Ridzuan Ahmad
Nov 29, 2011·Colloids and Surfaces. B, Biointerfaces·Hiroyuki ShintoKo Higashitani
Jun 15, 2011·Trends in Cell Biology·Daniel J Müller, Yves F Dufrêne
Aug 14, 2010·Experimental Cell Research·Cédric ZeltzYanusz Wegrowski
Feb 13, 2010·Ultramicroscopy·Federico Caneva SoumetzRoberto Raiteri
Oct 13, 2009·Journal of Biomechanics·Shamik Sen, Sanjay Kumar
Mar 7, 2009·Current Opinion in Biotechnology·Daniel J MüllerYves F Dufrêne
Jan 7, 2009·European Journal of Cell Biology·Marie-Marthe PhilippeauxJacques Mauël
Jan 8, 2008·Experimental Cell Research·Hugh KimChristopher A McCulloch
Aug 10, 2007·Journal of Molecular Biology·Jens FriedrichsDaniel J Muller
Dec 3, 2014·Developmental Biology·Jubin Kashef, Clemens M Franz
Sep 25, 2008·Cytometry. Part B, Clinical Cytometry·Arkadiusz PierzchalskiAttila Tárnok
Aug 4, 2011·Journal of Molecular Recognition : JMR·Małgorzata LekkaPiotr Laidler
Oct 4, 2013·Journal of Molecular Recognition : JMR·Lu DaoClemens M Franz
Jan 3, 2013·Journal of Cellular Physiology·Steven Frank KemenyAlisa Morss Clyne

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