Revealing of endogenous Marinobufagin by an ultra-specific and sensitive UHPLC-MS/MS assay in pregnant women

Talanta
Charline LenaertsBertrand Blankert

Abstract

Marinobufagenin (MBG) is a bufadienolide cardiac inotrope implicated in volume expansion-mediated hypertensive states including essential hypertension and preeclampsia (PE). Endogenous MBG is an inhibitor of the α1-isoform of Na+,K+-ATPase with vasoconstrictive and cardiotonic properties, causing hypertension and natriuresis. Elevated endogenous MBG-like material levels have been described by immunoassays in salt-sensitive pregnant and preeclamptic rats as well as in preeclamptic human patients. The rise of endogenous MBG-like material appears prior the development of the main symptoms of PE, leading us to consider MBG as one of the potential biomarkers for PE. The weak specificity and the high variability of the published immunoassays gives no certification about endogenous MBG existence. This led us to set-up a highly specific and sensitive analytical method to detect MBG in plasma at low levels relying on liquid chromatography combined to mass spectrometry (UHPLC-MS/MS) with recording of 7 highly specific MRM transitions for MBG. Pure MBG standard used in the method development was obtained by purification from the Bufo marinus toad venom. d3-25-hydroxyvitamin D3 was used as internal standard. An increasing organic gradient ...Continue Reading

References

Jan 23, 1991·Biochimica Et Biophysica Acta·D LichtsteinU Katz
Feb 14, 1995·European Journal of Pharmacology·A Y BagrovO V Fedorova
Aug 3, 1996·Lancet·P J HiltonW E Morden
Feb 14, 1998·The Journal of Biological Chemistry·R SchneiderW Schoner
Jun 11, 1998·Phytochemistry·L Krenn, B Kopp
Jul 31, 1998·Clinical and Experimental Hypertension : CHE·H C GonickO V Fedorova
Aug 31, 1999·Journal of Hypertension·D A LopatinA Y Bagrov
May 25, 2002·European Journal of Biochemistry·Wilhelm Schoner
Sep 6, 2005·Seminars in Nephrology·Wilhelm Schoner, Georgios Scheiner-Bobis
Sep 24, 2005·American Journal of Nephrology·Hop V VuJules B Puschett
Aug 21, 2007·Biochimica Et Biophysica Acta·Tatjana MijatovicRobert Kiss
Mar 12, 2009·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Darijana HorvatJules B Puschett
Mar 28, 2009·Pharmacological Reviews·Alexei Y BagrovOlga V Fedorova
Feb 20, 2010·Biochimica Et Biophysica Acta·J B PuschettM N Uddin
Mar 30, 2010·Biochimica Et Biophysica Acta·Olga V FedorovaAlexei Y Bagrov
Apr 15, 2010·The Journal of Biological Chemistry·Adriana KatzSteven J D Karlish
Jul 16, 2010·Clinical Biochemistry·Mark M KushnirA Wayne Meikle
Jan 22, 2011·Journal of Immunoassay & Immunochemistry·Daad Abi-GhanemJules B Puschett
Feb 5, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Elena V KolmakovaAlexei Y Bagrov
Mar 8, 2011·American Journal of Perinatology·Enoch AgunanneJules B Puschett
Jan 3, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Therese KoalManfred Rauh
Jun 12, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Mohammad N UddinThomas J Kuehl
Oct 23, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Alan H B Wu, Deborah French
Dec 18, 2013·Analytical and Bioanalytical Chemistry·Kelvin Sze-Yin Leung, Bonnie Mei-Wah Fong
Feb 11, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Silvia BaecherMichael Vogeser
Feb 21, 2014·Drugs·Catherine M Brown, Vesna D Garovic
May 3, 2014·Clinical and Experimental Hypertension : CHE·Andreas TomaschitzStefan Pilz
May 10, 2017·Journal of the American Society of Hypertension : JASH·Michael A Garfinkle

❮ Previous
Next ❯

Citations

Jan 23, 2019·Journal of Separation Science·Charline LenaertsBertrand Blankert
Aug 15, 2018·International Journal of Molecular Sciences·Olga V FedorovaAlexei Y Bagrov
Sep 12, 2018·International Journal of Molecular Sciences·Fatimah K KhalafDavid J Kennedy
Feb 25, 2020·Cardiology Research and Practice·Alejandro Ogazon Del ToroArturo Ponce
Mar 7, 2021·International Journal of Molecular Sciences·Natalia I AgalakovaAlexei Y Bagrov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.