Revealing the ability of a novel polysaccharide bioflocculant in bioremediation of heavy metals sensed in a Vibrio bioluminescence reporter assay

Environmental Pollution
Arya SajayanJoseph Selvin

Abstract

A bioflocculant-producing bacterial strain, designated MSI021, was isolated from the marine sponge Dendrilla nigra and demonstrated 94% flocculation activity in a kaolin clay suspension. MSI021 was identified as Bacillus cereus based on phylogenetic affiliation and biochemical characteristics. The purified extra-cellular bioflocculant was chemically elucidated as a polysaccharide molecule. The polysaccharide bioflocculant was stable under both acidic and alkaline conditions (pH 2.0-10.0) and temperatures up to 100 °C. The purified bioflocculant efficiently nucleated the formation of silver nanoparticles which showed broad spectrum antibacterial activity. The ability of the bioflocculant to remediate heavy metal toxicity was evaluated by measuring the inhibition of bioluminescence expression in Vibrio harveyi. Enrichment of heavy metals such as zinc, mercury and copper at concentrations of 1, 2 and 3 mM in culture media showed significant reduction of bioluminescence in Vibrio, whereas media enriched with heavy metals and bioflocculant showed dose dependent improvement in the expression of bioluminescence. The assay results demonstrated that the polysaccharide bioflocculant effectively mitigates heavy metal toxicity, thereby imp...Continue Reading

Citations

Mar 16, 2018·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Maiko K OkajimaTatsuo Kaneko
Sep 11, 2020·Materials·Piotr MaćczakMarta Ziegler-Borowska
Sep 22, 2019·Journal of Hazardous Materials·Patricia GiovanellaLara D Sette
Aug 13, 2021·Critical Reviews in Food Science and Nutrition·Maoping DuanTuo Zhang

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