Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging

Scientific Reports
Jing GaoHongda Wang

Abstract

Signal transducers and activators of transcription (STATs) can transduce cytokine signals and regulate gene expression. The cellular localization and nuclear trafficking of STAT1, a representative of the STAT family with multiple transcriptional functions, is tightly related with transcription process, which usually happens in the interphase of the cell cycle. However, these priority questions regarding STAT1 distribution and localization at the different cell-cycle stages remain unclear. By using direct stochastic optical reconstruction microscopy (dSTORM), we found that the nuclear expression level of STAT1 increased gradually as the cell cycle carried out, especially after EGF stimulation. Furthermore, STAT1 formed clusters in the whole cell during the cell cycle, with the size and the number of clusters also increasing significantly from G1 to G2 phase, suggesting that transcription and other cell-cycle related activities can promote STAT1 to form more and larger clusters for fast response to signals. Our work reveals that the cellular localization and clustering distribution of STAT1 are associated with the cell cycle, and further provides an insight into the mechanism of cell-cycle regulated STAT1 signal transduction.

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M J GutchN C Reich
Sep 12, 1997·Science·J E Darnell
Mar 26, 1999·European Journal of Biochemistry·M Köster, H Hauser
Jun 8, 1999·The Journal of Biological Chemistry·M A OlayioyeN E Hynes
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·A BegittU Vinkemeier
Oct 26, 2000·Trends in Biochemical Sciences·C M Horvath
Mar 15, 2001·Current Opinion in Cell Biology·J N Ihle
May 15, 2001·The EMBO Journal·B F LillemeierI M Kerr
Dec 13, 2001·Experimental Cell Research·Thomas MeyerUwe Vinkemeier
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·David E Levy, J E Darnell
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Jul 23, 2008·Angewandte Chemie·Mike HeilemannMarkus Sauer
Jun 6, 2009·Annual Review of Biochemistry·Bo HuangXiaowei Zhuang
Jan 9, 2010·Journal of Microscopy·S WolterM Sauer
Feb 12, 2010·Journal of Biophotonics·Dylan M OwenKatharina Gaus
May 1, 2010·Nature Methods·Ricardo HenriquesMusa M Mhlanga
Jul 21, 2010·The Journal of Cell Biology·Lothar SchermellehHeinrich Leonhardt
Nov 19, 2010·Cell Cycle·Gloria DimcoAnastasis Stephanou
May 4, 2011·Current Opinion in Chemical Biology·David KlenermanSimon J Davis
Sep 20, 2011·Nature Methods·Prabuddha SenguptaJennifer Lippincott-Schwartz
Dec 24, 2011·PLoS Pathogens·Martin LehmannVincent Piguet
Feb 1, 2012·Nature Methods·Ulrike SchnellBen N G Giepmans
Apr 10, 2012·Nature Methods·Dirk LandgrafJohan Paulsson

❮ Previous
Next ❯

Citations

Mar 25, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bo TengBinfeng Lu
Apr 4, 2017·Methods and Applications in Fluorescence·Jing GaoHongda Wang
Oct 29, 2020·Genome Génome / Conseil National De Recherches Canada·Rachel M PriceSheila S Teves
Oct 5, 2017·Analytical Chemistry·Hua HeFang Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
flow cytometry
fluorescence imaging
imaging techniques
nuclear translocation
gel filtration

Software Mentioned

quickPALM
Matlab
dSTORM
Image
Image J
Pro Plus

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.