Revealing the Dynamics of Thylakoid Membranes in Living Cyanobacterial Cells

Scientific Reports
Laura-Roxana StingaciuMichael Ohl

Abstract

Cyanobacteria are photosynthetic prokaryotes that make major contributions to the production of the oxygen in the Earth atmosphere. The photosynthetic machinery in cyanobacterial cells is housed in flattened membrane structures called thylakoids. The structural organization of cyanobacterial cells and the arrangement of the thylakoid membranes in response to environmental conditions have been widely investigated. However, there is limited knowledge about the internal dynamics of these membranes in terms of their flexibility and motion during the photosynthetic process. We present a direct observation of thylakoid membrane undulatory motion in vivo and show a connection between membrane mobility and photosynthetic activity. High-resolution inelastic neutron scattering experiments on the cyanobacterium Synechocystis sp. PCC 6803 assessed the flexibility of cyanobacterial thylakoid membrane sheets and the dependence of the membranes on illumination conditions. We observed softer thylakoid membranes in the dark that have three-to four fold excess mobility compared to membranes under high light conditions. Our analysis indicates that electron transfer between photosynthetic reaction centers and the associated electrochemical proton ...Continue Reading

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Citations

Apr 8, 2017·The Journal of Physical Chemistry Letters·Divina B AnunciadoHugh O'Neill
Apr 4, 2017·Nature Communications·Matej KandučEmanuel Schneck
Feb 27, 2017·Biochimica Et Biophysica Acta. Bioenergetics·Renáta ÜnnepGergely Nagy
Jan 4, 2019·Acta Crystallographica. Section D, Structural Biology·Rana AshkarJeremy C Smith
Feb 2, 2019·Geobiology·Emily D MatysRoger E Summons
Mar 10, 2018·Scientific Reports·Eugene Mamontov
Jul 22, 2020·Annual Review of Microbiology·Conrad W Mullineaux, Lu-Ning Liu
Dec 25, 2019·Physical Review. E·Arghya MajeeRudolf Podgornik
Oct 28, 2019·The Biochemical Journal·Petar H Lambrev, Parveen Akhtar
Dec 20, 2019·Scientific Reports·Dainius JakubauskasJacob J K Kirkensgaard
Dec 16, 2017·Plant Science : an International Journal of Experimental Plant Biology·Helmut Kirchhoff
May 7, 2021·Frontiers in Chemistry·Dainius JakubauskasJacob J K Kirkensgaard
Dec 11, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Shuo QianLuke A Clifton
Nov 10, 2021·Chemical Reviews·Yoo Kyung Go, Cecilia Leal

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Methods Mentioned

BETA
super-resolution
NMR
Small Angle Neutron Scattering
SANS
neutron spectroscopy
light scattering

Software Mentioned

ECHODET

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