Reversal of adipose tissue loss by probucol in mice with deficiency of both scavenger receptor class B type 1 and LDL receptor on high fat diet

Biochemical and Biophysical Research Communications
Xin GuoGeorge Liu

Abstract

Scavenger receptor class B type 1(SR-B1) and low density lipoprotein receptor (LDLR) play vital roles in cholesterol homeostasis. Previous studies indicated a strong link between cholesterol and adipose tissue (AT). In this study, adult male SR-B1 and LDLR double knockout (DKO) mice were fed with high fat diet (HFD) for 3 months. Interestingly, we found severe loss of AT in DKO mice fed with HFD. To reverse the AT loss in DKO mice, 1% probucol was added in HFD. In DKO mice on HFD, plasma total cholesterol (TC) and free cholesterol (FC) levels were increased 6 and 15 folds respectively compared with wild type (WT) mice. We found severe loss of AT in whole body of DKO mice compared with WT or single KO mice. In AT of DKO mice, histology showed the very small size of adipocytes and infiltration of inflammatory cells; Genes expressions related to fatty acid uptake, lipogenesis and adipogenesis were decreased; TUNEL analysis and related genes expressions of endoplasmic reticulum (ER) stress and inflammation were significantly higher than those of WT or single KO mice. Probucol could reduce increased TC and FC levels, and reverse the loss of fat and apoptosis of AT in DKO mice. AT loss in DKO mice with HFD was probably due to high le...Continue Reading

Citations

Sep 15, 2020·Chinese Neurosurgical Journal·Cameron LenahanJohn H Zhang
Oct 13, 2019·Atherosclerosis·Markus AxmannHerbert Stangl
Feb 26, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Katherine RiveraAttilio Rigotti
Oct 26, 2021·Frontiers in Cardiovascular Medicine·Jiawei LiaoYunlong Xia

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