Reversal of carbapenem-resistance in Shewanella algae by CRISPR/Cas9 genome editing

Journal of Advanced Research
Zong-Yen WuPo-Yu Liu

Abstract

Antibiotic resistance in pathogens is a growing threat to human health. Of particular concern is resistance to carbapenem, which is an antimicrobial agent listed as critically important by the World Health Organization. With the global spread of carbapenem-resistant organisms, there is an urgent need for new treatment options. Shewanella algae is an emerging pathogen found in marine environments throughout the world that has increasing resistance to carbapenem. The organism is also a possible antibiotic resistance reservoir in humans and in its natural habitat. The development of CRISPR/Cas9-based methods has enabled precise genetic manipulation. A number of attempts have been made to knock out resistance genes in various organisms. The study used a single plasmid containing CRISPR/Cas9 and recE/recT recombinase to reverse an antibiotic-resistant phenotype in S. algae and showed blaOXA-55-like gene is essential for the carbapenem resistance. This result demonstrates a potential validation strategy for functional genome annotation in S. algae.

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Citations

Aug 27, 2019·The Journal of General and Applied Microbiology·Yusuke SuzukiKazuya Watanabe
May 6, 2020·Infection and Drug Resistance·Peng WanZhenling Zeng
Apr 10, 2020·Ci Ji Yi Xue Za Zhi = Tzu-chi Medical Journal·Jui-Hsing WangKwong-Chung Tung
Nov 26, 2020·BMC Microbiology·Vinicius Buiatte de Andrade AlvesVera Letticie de Azevedo Ruiz
Jun 9, 2020·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Chien-Hao TsengPo-Yu Liu

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Datasets Mentioned

BETA
CP032414

Methods Mentioned

BETA
PCR
gene knockout

Software Mentioned

BLAST
SMRT Analysis portal
RPSBLAST
Canu

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