Reversal of myoblast aging by tocotrienol rich fraction posttreatment

Oxidative Medicine and Cellular Longevity
Jing Jye LimNorwahidah Abdul Karim

Abstract

Skeletal muscle satellite cells are heavily involved in the regeneration of skeletal muscle in response to the aging-related deterioration of the skeletal muscle mass, strength, and regenerative capacity, termed as sarcopenia. This study focused on the effect of tocotrienol rich fraction (TRF) on regenerative capacity of myoblasts in stress-induced premature senescence (SIPS). The myoblasts was grouped as young control, SIPS-induced, TRF control, TRF pretreatment, and TRF posttreatment. Optimum dose of TRF, morphological observation, activity of senescence-associated β-galactosidase (SA-β-galactosidase), and cell proliferation were determined. 50 μg/mL TRF treatment exhibited the highest cell proliferation capacity. SIPS-induced myoblasts exhibit large flattened cells and prominent intermediate filaments (senescent-like morphology). The activity of SA-β-galactosidase was significantly increased, but the proliferation capacity was significantly reduced as compared to young control. The activity of SA-β-galactosidase was significantly reduced and cell proliferation was significantly increased in the posttreatment group whereas there was no significant difference in SA-β-galactosidase activity and proliferation capacity of pretrea...Continue Reading

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Citations

Oct 3, 2015·Nutrition·Michael J Glade, Michael M Meguid
Aug 8, 2015·Ageing Research Reviews·Anabela La CollaAndrea Vasconsuelo
Aug 7, 2014·Oxidative Medicine and Cellular Longevity·Shy Cian KhorSuzana Makpol
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Mar 21, 2019·International Journal of Molecular Sciences·Kok-Yong Chin, Soelaiman Ima-Nirwana
Sep 12, 2021·Bone Research·Mei WanJennifer H Elisseeff

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Methods Mentioned

BETA
biopsies
biopsy

Software Mentioned

SPSS Statistics

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