Reversal of myofibroblasts by amniotic membrane stromal extract

Journal of Cellular Physiology
Wei LiScheffer C G Tseng

Abstract

Myofibroblasts play an important role in morphogenesis, inflammation, and fibrosis in most tissues. The amniotic membrane stroma can maintain keratocytes in cultures and prevent them from differentiating into myofibroblasts. However, it is unknown whether the AM stroma can also reverse differentiated myofibroblasts. In this study, we found that amniotic membrane stromal cells (AMSCs), which adopted fibroblastic phenotype in vivo, quickly and completely differentiated into myofibroblasts during ex vivo culture in DMEM/FBS on plastic within 2 passages. When cultured on type I collagen, the myofibroblasts maintained their phenotype, however, when these myofibroblasts were re-seeded onto a cryopreserved amniotic membrane stromal surface, they reversed to the fibroblast phenotype. Moreover, we found that the amniotic membrane stromal extract not only helps maintain primary AMSCs fibroblastic phenotype in vitro, but also can reverse differentiated myofibroblasts back to fibroblasts. This reversal was not coupled with cell proliferation. We concluded that the amniotic membrane stroma contains soluble factors that can regulate the mesenchymal cell differentiation. Further investigation into the identity of these factors and the control...Continue Reading

References

Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·S K MasurS Petridou
Dec 1, 1998·The Journal of Physiology·D W Powell
Jan 5, 1999·Journal of Neuroscience Methods·C N SvendsenM A Caldwell
Aug 17, 2001·Current Opinion in Ophthalmology·K C SippelC S Foster
May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
Jun 17, 2003·Cell Structure and Function·Mitsuru SatoHaruki Senoo
Jul 8, 2003·The Journal of Pathology·G Gabbiani
Oct 29, 2003·Current Opinion in Biotechnology·Boris Hinz, Giulio Gabbiani
Nov 12, 2003·Survey of Ophthalmology·Martin GrueterichScheffer C G Tseng
Nov 26, 2003·Investigative Ophthalmology & Visual Science·Edgar M EspanaScheffer C G Tseng
Jan 9, 2004·Survey of Ophthalmology·Harminder S DuaV Senthil Maharajan
Aug 25, 2004·Investigative Ophthalmology & Visual Science·Edgar M EspanaScheffer C G Tseng
Apr 27, 2005·Investigative Ophthalmology & Visual Science·Seiichi YokooShiro Amano
Jul 30, 2005·Stem Cells·Yiqin DuJames L Funderburgh
Mar 28, 2006·Investigative Ophthalmology & Visual Science·Tobias Meyer-Ter-VehnPetra Knaus
Apr 28, 2006·Investigative Ophthalmology & Visual Science·Tetsuya KawakitaScheffer C G Tseng

❮ Previous
Next ❯

Citations

Mar 9, 2013·American Journal of Respiratory Cell and Molecular Biology·Garth GarrisonMarc Peters-Golden
Jun 21, 2013·Fibrogenesis & Tissue Repair·Ariel Bing-Shi TanMichael Raghunath
Mar 26, 2009·Regenerative Medicine·Ornella ParoliniDörthe Schmidt
Apr 20, 2014·European Journal of Pharmacology·XiaoHong YangXiaoHong Chen
May 7, 2013·Cytotherapy·Patricia VosdoganesEuan M Wallace
Feb 23, 2011·Differentiation; Research in Biological Diversity·Silvia Díaz-PradoFrancisco J Blanco
Jul 17, 2015·Clinical & Experimental Ophthalmology·Peter J Wilson, Trevor Sherwin
Dec 17, 2014·Clinical & Experimental Ophthalmology·David V DudokCindy M Hutnik
Aug 27, 2014·Cytotherapy·Dandan ZhuRebecca Lim
May 1, 2012·American Journal of Obstetrics and Gynecology·Ryan J HodgesEuan M Wallace
May 12, 2012·Stem Cells International·Ryan J HodgesEuan M Wallace
Apr 26, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yo KishimotoNathan V Welham
Oct 8, 2014·Cell Transplantation·Gary Hin-Fai YamJodhbir S Mehta
Oct 10, 2012·Cell Transplantation·Patricia VosdoganesRebecca Lim
May 31, 2017·Clinical Spine Surgery : a Spine Publication·D Greg AndersonJulie O'Connell
Dec 7, 2018·Expert Opinion on Biological Therapy·Jan AdamowiczTomasz Drewa
Sep 13, 2018·Journal of Cellular Physiology·Michele RiccioFrancesco De Francesco
Feb 2, 2019·Investigative Ophthalmology & Visual Science·Xin CheWei Li
Mar 18, 2011·American Journal of Physiology. Cell Physiology·Yehui TanWei Li
Jul 9, 2020·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Megan S LordJohn M Whitelock
Sep 13, 2015·Experimental Biology and Medicine·Meirong LiXiaobing Fu
Aug 23, 2020·Journal of Clinical Medicine·Tomaso CaporossiStanislao Rizzo
Apr 21, 2020·Taiwan Journal of Ophthalmology·Sean TigheScheffer C G Tseng
Apr 29, 2020·Ophthalmology Retina·Stanislao RizzoGianni Virgili
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Fatemeh Dadkhah TehraniAzadeh Shabani
Aug 17, 2020·Experimental Eye Research·Scheffer C G TsengSean Tighe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.