PMID: 153405Dec 8, 1978

Reversal of the calcium pump in human red cells

The Journal of Membrane Biology
J P RossiA F Rega

Abstract

Human red cells containing low ATP and high Pi concentrations were suspended in media with and without 2 mM Ca2+, and the incorporation of (32P)Pi into ATP was measured. There was some incorporation whatever the medium, but in every experiment there was an extra incorporation when the cells were in the Ca2+-containing medium. This extra incorporation was abolished by the ionophore A23187, which collapses the Ca2+ concentration gradient across the membranes, or by LaCl3, which blocks the Ca2+ pump. Starved and phosphate-loaded cells also show an uptake of Ca2+ which is not apparent in fresh cells. Results are consistent with the idea that Ca2+-dependent incorporation of Pi into ATP is catalyzed by the Ca2+ pump using energy derived from the Ca2+ concentration gradient.

References

Feb 2, 1978·Biochimica Et Biophysica Acta·A F Rega, P J Garrahan
Aug 4, 1978·Biochimica Et Biophysica Acta·D E RichardsP J Garrahan
Jun 30, 1977·The Journal of Membrane Biology·D E RichardsP J Garrahan
Mar 1, 1976·The Journal of Physiology·T J Simons
Apr 1, 1969·The Journal of Physiology·H J Schatzmann, F F Vincenzi
Jun 1, 1973·The Journal of Physiology·R P Garay, P J Garrahan
Sep 1, 1964·The Journal of General Physiology·D SAVITZA K SOLOMON
Dec 1, 1971·The Journal of Membrane Biology·R M Blum, J F Hoffman

Citations

Apr 1, 1994·Diabetes/metabolism Reviews·J J Gagliardino, J P Rossi
Dec 15, 1979·Experientia·A WüthrichP Romero
Nov 6, 1981·Biochimica Et Biophysica Acta·J P RossiA F Rega
Sep 30, 1980·Biochimica Et Biophysica Acta·B Sarkadi
Jan 1, 1980·Annals of Biomedical Engineering·P C Chen

Related Concepts

Calcimycin
Ca(2+)-Transporting ATPase
Striadyne
Calcium
Erythrocytes
Iodoacetamide
Lanthanum
Orthophosphate
Thermodynamics
In Vitro [Publication Type]

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