Reversal of the Caspase-Dependent Apoptotic Cytotoxicity Pathway by Taurine from Lycium barbarum (Goji Berry) in Human Retinal Pigment Epithelial Cells: Potential Benefit in Diabetic Retinopathy.

Evidence-based Complementary and Alternative Medicine : ECAM
M K SongT H W Huang

Abstract

Diabetic retinopathy is a preventable microvascular diabetic complication and a leading cause of vision loss. Retinal pigment epithelial cell apoptosis is an early event in diabetic retinopathy. Taurine is reportedly beneficial for diabetic retinopathy and is abundant in the fruit of Lycium barbarum (LB). We have investigated the effect of pure taurine and an extract of LB rich in taurine on a model of diabetic retinopathy, the retinal ARPE-19 cell line exposed to high glucose. We demonstrate for the first time that LB extract and the active ligand, taurine, dose dependently enhance cell viability following high glucose treatment in the ARPE-19 retinal epithelial cell line. This cytoprotective effect was associated with the attenuation of high glucose-induced apoptosis, which was shown by characteristic morphological staining and the dose-dependent decrease in the number of apoptotic cells, determined by flow cytometry. Moreover, we have shown that LB extract and taurine dose dependently downregulate caspase-3 protein expression and the enzymatic activity of caspase-3. We conclude that taurine, a major component of LB, and the LB extract, have a cytoprotective effect against glucose exposure in a human retinal epithelial cell l...Continue Reading

References

Feb 1, 1996·Experimental Eye Research·K C DunnL M Hjelmeland
Dec 1, 1995·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·E O Hoffmann
Jan 1, 1995·Eye·C J KennedyI J Constable
Mar 1, 1997·Journal of Holistic Nursing : Official Journal of the American Holistic Nurses' Association·C A Beckmann
Dec 22, 1999·The American Journal of Physiology·Q D WuD Bouchier-Hayes
Jun 29, 2000·Annual Review of Biochemistry·W C EarnshawS H Kaufmann
Aug 6, 2000·Cell and Tissue Research·C Stadelmann, H Lassmann
Sep 15, 2000·Journal of Immunological Methods·I VermesC Reutelingsperger
Oct 18, 2000·Survey of Ophthalmology·S BeattyM Boulton
Dec 18, 2001·Diabetes/metabolism Research and Reviews·S H Hansen
Dec 26, 2001·The Journal of Pharmacology and Experimental Therapeutics·Gerald F DaviesWilliam J Roesler
Nov 6, 2002·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Yoshinori AizuHachiro Nakagawa
Jan 31, 2003·Journal of Perinatology : Official Journal of the California Perinatal Association·Tina Young, Robert E Shaddy
Mar 3, 2004·Neurochemical Research·Flavia FranconiGiovanni Ghirlanda
Mar 17, 2004·Biochemical and Biophysical Research Communications·Tomoka TakataniJunichi Azuma
Jul 28, 2004·Investigative Ophthalmology & Visual Science·Ahmed M Abu-El-AsrarKarel Geboes
Sep 29, 2004·Diabetes Care·Donald S FongRonald Klein
Feb 8, 2005·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·H ZhaoK Bojanowski
Sep 20, 2005·The Journal of Nutritional Biochemistry·David A AllenSteven M Harwood
Dec 17, 2005·Antioxidants & Redox Signaling·Renu A Kowluru
Dec 31, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Teng-Nan LinKenneth K Wu
Jan 31, 2006·Current Opinion in Clinical Nutrition and Metabolic Care·Flavia FranconiGiuseppe Seghieri
Mar 28, 2006·Investigative Ophthalmology & Visual Science·Renu A KowluruYeh-Shih Ho
Mar 6, 2007·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yuko KomaseToshihito Shinagawa
Aug 22, 2007·Cellular and Molecular Neurobiology·Raymond Chuen-Chung Chang, Kwok-Fai So
Feb 7, 2008·Apoptosis : an International Journal on Programmed Cell Death·Raphaël Roduit, Daniel F Schorderet
Feb 12, 2008·TheScientificWorldJournal·Jennifer L Wilkinson-Berka, Antonia G Miller
Feb 22, 2008·PPAR Research·Alexandra A HerzlichChi-Chao Chan
Apr 17, 2008·Diabetologia·A DecaniniD A Ferrington
Jan 28, 2009·Pharmacotherapy·Tayyeba K Ali, Azza B El-Remessy
Feb 20, 2009·Journal of Neurochemistry·Francesca DoonanThomas G Cotter
May 1, 2009·American Journal of Physiology. Renal Physiology·Heitham T HassounHamid Rabb

❮ Previous
Next ❯

Citations

Mar 24, 2012·Journal of Agricultural and Food Chemistry·Shiwei ZhangGuowu Yang
Apr 2, 2016·Neuromolecular Medicine·Xiwen XingKwok Fai So
Sep 18, 2013·Journal of Ethnopharmacology·Randhir SinghGirish Kumar Gupta
Dec 12, 2012·Journal of Agricultural and Food Chemistry·Xiaoqing LiuGuowu Yang
Jan 27, 2017·The Journal of Pharmacy and Pharmacology·Tapan Behl, Anita Kotwani
Apr 17, 2019·Nutrients·Maria Grazia Rossino, Giovanni Casini
Nov 21, 2014·Journal of Diabetes Investigation·Nam H ChoChol Shin
Jan 21, 2021·Nutrients·Kumari NeelamKah-Guan Au Eong

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
X-ray

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis