Reverse and conventional chemical ecology approaches for the development of oviposition attractants for Culex mosquitoes.

PloS One
Walter S LealAndré Furtado

Abstract

Synthetic mosquito oviposition attractants are sorely needed for surveillance and control programs for Culex species, which are major vectors of pathogens causing various human diseases, including filariasis, encephalitis, and West Nile encephalomyelitis. We employed novel and conventional chemical ecology approaches to identify potential attractants, which were demonstrated in field tests to be effective for monitoring populations of Cx. p. quinquefasciatus in human dwellings. Immunohistochemistry studies showed that an odorant-binding protein from this species, CquiOBP1, is expressed in trichoid sensilla on the antennae, including short, sharp-tipped trichoid sensilla type, which house an olfactory receptor neuron sensitive to a previously identified mosquito oviposition pheromone (MOP), 6-acetoxy-5-hexadecanolide. CquiOBP1 exists in monomeric and dimeric forms. Monomeric CquiOBP1 bound MOP in a pH-dependent manner, with a change in secondary structure apparently related to the loss of binding at low pH. The pheromone antipode showed higher affinity than the natural stereoisomer. By using both CquiOBP1 as a molecular target in binding assays and gas chromatography-electroantennographic detection (GC-EAD), we identified nonana...Continue Reading

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Citations

Apr 30, 2014·Journal of the American Mosquito Control Association·S R IrishM M Cameron
Feb 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yuko IshidaToshimasa Yamazaki
Aug 26, 2011·Journal of Insect Science·José G Crespo
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Jan 18, 2013·PloS One·Yuko IshidaWalter S Leal
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Jan 10, 2018·Proceedings of the National Academy of Sciences of the United States of America·Young-Moo ChooWalter S Leal

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Methods Mentioned

BETA
ion-exchange
gel filtration
circular dichroism
PCR
ion-exchange chromatography
electrophoresis
Fluorescence

Software Mentioned

EXPASY

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