Reverse enzyme immunoassay for detection of specific anti-Toxoplasma immunoglobulin M antibodies.

Journal of Clinical Microbiology
E L FrancoA J Sulzer

Abstract

A reverse enzyme immunoassay (R-EIA) is described, in which polystyrene muplates are sensitized with anti-immunoglobulin M (IgM) (mu chain) antibodies and then sequentially allowed to react with patient's serum, peroxidase-labeled Toxoplasma gondii soluble antigen, and substrate. Measurement of activity of the solid-phase bound enzyme conjugate was done by colorimetric reading of the final developed color and kinetically by the initial rate of color development. This R-EIA allowed full resolution between absorbance values of a group of 36 sera which presented positive results in the Toxoplasma IgM immunofluorescence test and the remaining groups, which consisted of 39 normal individuals, 22 rheumatoid factor-positive sera, 8 Waldenstrom's macroglobulinemic sera, 3 infectious mononucleosis samples, and 6 high-titered IgG anti-T. gondii sera. No interference of rheumatoid factor IgM or inhibition by high-titered specific IgG was detected, even in the false IgM immunofluorescence-positive rheumatoid factor samples. Likewise, false-negative IgM immunofluorescence samples gave positive R-EIA even without adsorption with Staphylococcus aureus protein A. The possibility of direct tagging of the antigen with the enzyme eliminates the n...Continue Reading

References

Sep 23, 1978·Lancet·W Duermeyer, J van der Veen
Sep 1, 1979·Scandinavian Journal of Clinical and Laboratory Investigation·B Dinesen, S Nielsen
Aug 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A J O'Beirne, H R Cooper
Feb 1, 1976·Journal of Clinical Pathology·A VollerB Oladehin
Apr 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·B HydeJ S Remington
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Jun 1, 1980·Journal of Clinical Microbiology·T M LinG R O'Connor

❮ Previous
Next ❯

Citations

Dec 1, 1984·European Journal of Clinical Microbiology·P PoulettyJ Kadouche
Jan 1, 1986·Revista Do Instituto De Medicina Tropical De São Paulo·J R MineoG Almeida
Aug 1, 1983·Journal of Clinical Microbiology·J B MahonyM A Chernesky
Apr 15, 1983·Journal of Immunological Methods·A BonioloM Zannino
Jun 1, 1997·Journal of Clinical Microbiology·M ArcaviG Griemberg
Dec 1, 1987·Journal of Clinical Microbiology·M WilsonK W Walls

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.