Abstract
A reverse enzyme immunoassay (R-EIA) is described, in which polystyrene muplates are sensitized with anti-immunoglobulin M (IgM) (mu chain) antibodies and then sequentially allowed to react with patient's serum, peroxidase-labeled Toxoplasma gondii soluble antigen, and substrate. Measurement of activity of the solid-phase bound enzyme conjugate was done by colorimetric reading of the final developed color and kinetically by the initial rate of color development. This R-EIA allowed full resolution between absorbance values of a group of 36 sera which presented positive results in the Toxoplasma IgM immunofluorescence test and the remaining groups, which consisted of 39 normal individuals, 22 rheumatoid factor-positive sera, 8 Waldenstrom's macroglobulinemic sera, 3 infectious mononucleosis samples, and 6 high-titered IgG anti-T. gondii sera. No interference of rheumatoid factor IgM or inhibition by high-titered specific IgG was detected, even in the false IgM immunofluorescence-positive rheumatoid factor samples. Likewise, false-negative IgM immunofluorescence samples gave positive R-EIA even without adsorption with Staphylococcus aureus protein A. The possibility of direct tagging of the antigen with the enzyme eliminates the n...Continue Reading
References
Sep 23, 1978·Lancet·W Duermeyer, J van der Veen
Apr 15, 1978·Lancet·J S Remington
Sep 1, 1979·Scandinavian Journal of Clinical and Laboratory Investigation·B Dinesen, S Nielsen
Mar 1, 1977·Journal of Clinical Microbiology·K W WallsD K English
Jul 1, 1978·Infection and Immunity·M E CamargoO S Nakahara
Aug 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A J O'Beirne, H R Cooper
Jan 1, 1979·Journal of Medical Virology·W H Gerlich, W Lüer
Jan 1, 1976·Journal of Immunological Methods·S ChantlerB A Hurn
Jun 1, 1977·Journal of Clinical Microbiology·R Handsher, A Fogel
Feb 1, 1976·Journal of Clinical Pathology·A VollerB Oladehin
Jan 1, 1975·Journal of Clinical Pathology·K A Karim, G B Ludlam
Apr 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·B HydeJ S Remington
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Jan 1, 1980·Journal of Medical Virology·M RoggendorfR Scheid
Jun 1, 1980·Journal of Clinical Microbiology·T M LinG R O'Connor
Feb 1, 1980·Infection and Immunity·J R MineoA W Ferreira
Citations
Dec 1, 1984·European Journal of Clinical Microbiology·P PoulettyJ Kadouche
Dec 1, 1989·Journal of Clinical Pathology·C VerhofstedeJ Plum
Jan 1, 1983·Journal of Immunoassay·E L FrancoJ C Soto
Jan 1, 1986·Revista Do Instituto De Medicina Tropical De São Paulo·J R MineoG Almeida
Aug 1, 1983·Journal of Clinical Microbiology·J B MahonyM A Chernesky
Apr 15, 1983·Journal of Immunological Methods·A BonioloM Zannino
Aug 1, 1982·Journal of Clinical Microbiology·H Schmitz
Jun 1, 1997·Journal of Clinical Microbiology·M ArcaviG Griemberg
Dec 1, 1987·Journal of Clinical Microbiology·M WilsonK W Walls
Dec 17, 1997·Journal of Clinical Microbiology·M WilsonD Ware
Jan 1, 1986·Journal of Clinical Microbiology·T M LinJ M Joseph
Jun 1, 1983·Journal of Clinical Microbiology·A M van LoonJ van der Veen
Feb 1, 1983·Journal of Clinical Microbiology·E Konishi, J Takahashi