PMID: 6539574Mar 1, 1984Paper

Reverse fibrin autography: a method to detect and partially characterize protease inhibitors after sodium dodecyl sulfate--polyacrylamide gel electrophoresis

Analytical Biochemistry
L A EricksonD J Loskutoff

Abstract

A new technique, reverse fibrin autography, was developed to detect protease inhibitors previously fractionated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Exogenous proteases were incorporated into fibrin-agar indicator films, eventually causing the fibrin to lyse. When an acrylamide gel containing inhibitors was placed on top of such an indicator, the positions of the inhibitors were revealed by the formation of opaque, lysis-resistant zones in the otherwise cleared fibrin film. The technique was versatile in that a variety of inhibitors were revealed, and semiquantitative since the size of the lysis-resistant zone in the indicator increased in proportion to the amount of inhibitor subjected to electrophoresis. This approach could be used not only to detect inhibitors having different protease specificities, but also to distinguish between the inhibitor activities of antibodies directed against urokinase or tissue-type plasminogen activator. Thus, reverse fibrin autography offers a convenient new approach to rapidly screen and partially characterize inhibitors present in complex biological samples.

References

Jul 1, 1978·The Journal of Experimental Medicine·A Granelli-Piperno, E Reich
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Citations

Oct 1, 1990·Journal of Cellular Physiology·E H AllanT J Martin
Jan 1, 1990·Histochemistry·P KristensenK Danø
Dec 30, 1999·In Vitro Cellular & Developmental Biology. Animal·J OhanL Drouet
Jan 1, 1992·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·R M StewartD R Morrison
Jan 2, 1990·Molecular and Cellular Endocrinology·P A AndreasenS N Stacey
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L A EricksonD J Loskutoff
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y SakataD J Loskutoff
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T NyD J Loskutoff
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J C KirchheimerB R Binder
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J F CajotB Sordat
Jul 1, 1985·Journal of Clinical Pathology·N A BoothB Bennett
Oct 1, 1984·The Journal of Clinical Investigation·L A EricksonD J Loskutoff
Aug 1, 1986·The Journal of Clinical Investigation·M P BevilacquaD J Loskutoff
Sep 1, 1995·The Journal of Clinical Investigation·M A OlmanD J Loskutoff
Aug 5, 2010·Thrombosis Research·Kurosh ParsiJoanne Emily Joseph
Aug 15, 1985·Thrombosis Research·M ShimonakaY Inada
Nov 1, 1987·Thrombosis Research·J C KirchheimerB R Binder

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