Reverse genetics of Escherichia coli glycerol kinase allosteric regulation and glucose control of glycerol utilization in vivo

Journal of Bacteriology
C K HoltmanD W Pettigrew

Abstract

Reverse genetics is used to evaluate the roles in vivo of allosteric regulation of Escherichia coli glycerol kinase by the glucose-specific phosphocarrier of the phosphoenolpyruvate:glycose phosphotransferase system, IIA(Glc) (formerly known as III(glc)), and by fructose 1,6-bisphosphate. Roles have been postulated for these allosteric effectors in glucose control of both glycerol utilization and expression of the glpK gene. Genetics methods based on homologous recombination are used to place glpK alleles with known specific mutations into the chromosomal context of the glpK gene in three different genetic backgrounds. The alleles encode glycerol kinases with normal catalytic properties and specific alterations of allosteric regulatory properties, as determined by in vitro characterization of the purified enzymes. The E. coli strains with these alleles display the glycerol kinase regulatory phenotypes that are expected on the basis of the in vitro characterizations. Strains with different glpR alleles are used to assess the relationships between allosteric regulation of glycerol kinase and specific repression in glucose control of the expression of the glpK gene. Results of these studies show that glucose control of glycerol ut...Continue Reading

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Feb 7, 2001·Journal of Bacteriology·C K HoltmanD W Pettigrew
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Citations

May 10, 2011·The Journal of Biological Chemistry·M Kenyon ApplebeeBernhard Ø Palsson
Jul 30, 2009·Nucleic Acids Research·Yoshinao AzumaMutsunori Shirai
Oct 3, 2006·Journal of Bacteriology·Andrew R JoyceSanjay Agarwalla
May 11, 2002·Journal of Bacteriology·Tanja EpplerWinfried Boos
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Aug 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aaron C Pawlyk, Donald W Pettigrew
Aug 24, 2013·Environmental Microbiology·Pablo I NikelVíctor de Lorenzo
Oct 22, 2016·Environmental Microbiology·Timothy J WilliamsRicardo Cavicchioli
Nov 3, 2005·The Journal of Biological Chemistry·Katja BettenbrockErnst-Dieter Gilles
Mar 12, 2020·World Journal of Microbiology & Biotechnology·Chen SongXue-Hong Zhang
Dec 20, 2020·International Journal of Molecular Sciences·Piotr WilkPrzemysław Grudnik
Sep 30, 2021·Microbiology and Molecular Biology Reviews : MMBR·Erin F BoulangerBrian M M Ahmer

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