Reverse Genetics of Influenza B Viruses

Methods in Molecular Biology
Aitor NogalesLuis Martínez-Sobrido

Abstract

Annual influenza epidemics are caused not only by influenza A viruses but also by influenza B viruses. Initially established for the generation of recombinant influenza A viruses, plasmid-based reverse genetics techniques have allowed researchers the generation of wild type and mutant viruses from full-length cDNA copies of the influenza viral genome. These reverse genetics approaches have allowed researchers to answer important questions on the biology of influenza viruses by genetically engineering infectious recombinant viruses. This has resulted in a better understanding of the molecular biology of influenza viruses, including both viral and host factors required for genome replication and transcription. With the ability to generate recombinant viruses containing specific mutations in the viral genome, these reverse genetics tools have also allowed the identification of viral and host factors involved in influenza pathogenesis, transmissibility, host-range interactions and restrictions, and virulence. Likewise, reverse genetics techniques have been used for the implementation of inactivated or live-attenuated influenza vaccines and the identification of anti-influenza drugs and their mechanism of antiviral activity. In 2002...Continue Reading

Citations

Aug 14, 2019·Viruses·Rebekah E Dumm, Nicholas S Heaton
Jan 11, 2020·Frontiers in Microbiology·Aitor NogalesLuis Martínez-Sobrido
Dec 9, 2020·Antimicrobial Agents and Chemotherapy·Evelien VanderlindenDominique Schols
Mar 16, 2021·Frontiers in Microbiology·Nicolas MalausseSandie Munier
May 1, 2021·Viruses·Aitor NogalesLuis Martinez-Sobrido
Jul 6, 2021·Virus Research·Sarah CaddyUlrich Desselberger

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