Reverse Genetics of Zika Virus

Methods in Molecular Biology
Chao ShanPei-Yong Shi

Abstract

We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A T7 promoter was engineered in front of the viral 5' UTR for in vitro transcription. A hepatitis delta virus ribozyme (HDVr) sequence was engineered following the viral 3' UTR for generation of the authentic 3' end of the RNA transcript.

Citations

Dec 22, 2017·The Journal of Infectious Diseases·Xuping XiePei-Yong Shi
Jan 31, 2018·Nature Communications·Hongjie XiaPei-Yong Shi
Jun 22, 2020·Current Opinion in Virology·Camila R Fontes-GarfiasPei-Yong Shi
Mar 16, 2021·Frontiers in Microbiology·Iasmim Silva de MelloMyrna Cristina Bonaldo
Aug 7, 2021·Emerging Microbes & Infections·Krisangel LópezAlbert J Auguste
Nov 9, 2021·Frontiers in Microbiology·Zhiran QinWei Zhao

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