Reverse genetics system for introduction of site-specific mutations into the double-stranded RNA genome of infectious rotavirus

Proceedings of the National Academy of Sciences of the United States of America
Satoshi KomotoKoki Taniguchi

Abstract

We describe here the successful establishment of a reverse genetics system for rotavirus (RV), a member of the Reoviridae family whose genome consists of 10-12 segmented dsRNA. The system is based on the recombinant vaccinia virus T7 RNA polymerase-driven procedure for supplying artificial viral mRNA in the cytoplasm. With the aid of helper virus (human RV strain KU) infection, intracellularly transcribed full-length VP4 mRNA of simian RV strain SA11 resulted in the rescue of the KU-based transfectant virus carrying the SA11 VP4 RNA segment derived from cDNA. In addition to the rescued transfectant virus with the authentic SA11 VP4 gene, three more infectious RV transfectants, into which silent mutation(s) were introduced to destroy both or one of the two restriction enzyme sites as gene markers in the SA11 VP4 genome, were also rescued with this method. The ability to artificially manipulate the RV genome will greatly increase the understanding of the replication and the pathogenicity of RV and will provide a tool for the design of attenuated vaccine vectors.

References

Nov 1, 1991·Virology·M EnamiP Palese
Dec 1, 1990·Virology·M R RonerW K Joklik
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V M OlkkonenL Mindich
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M EnamiP Palese
Jan 1, 1986·Journal of Virology·P A OffitH F Clark
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Apr 15, 1983·Virology·M A McCrae, J G McCorquodale
Nov 1, 1994·Journal of Virology·D ChenR F Ramig
Jun 1, 1996·Journal of Virology·J T PattonR F Ramig
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·E Mundt, V N Vakharia
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·P PaleseA García-Sastre
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·M R RonerW K Joklik
Dec 3, 1999·Advances in Virus Research·D J Evans
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·M R Roner, W K Joklik
Aug 21, 2002·Journal of Virology·J M DiproseD I Stuart
May 10, 2003·Emerging Infectious Diseases·Umesh D ParasharRoger I Glass
Aug 10, 2004·Current Topics in Microbiology and Immunology·K K Conzelmann
Aug 10, 2004·Current Topics in Microbiology and Immunology·G Neumann, Y Kawaoka

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Citations

Jul 16, 2008·Veterinary Research Communications·K DhamaS V S Malik
Dec 17, 2009·Nature Biotechnology·Eckard WimmerJeffery K Taubenberger
Jun 16, 2007·Nature Reviews. Microbiology·Juana AngelHarry B Greenberg
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shane D TraskJohn T Patton
Feb 24, 2009·British Medical Bulletin·Ulrich DesselbergerJim Gray
Jul 29, 2006·Journal of Virology·Zenobia F TaraporewalaJohn T Patton
Apr 30, 2010·Journal of Virology·Cécile TroupinAntoine Garbarg-Chenon
Sep 15, 2006·Journal of Virology·Shane D Trask, Philip R Dormitzer
Dec 8, 2006·Journal of Virology·Mark Boyce, Polly Roy
Nov 24, 2006·Journal of Virology·Steven R AllenGregory M Tiao
Apr 30, 2009·Annual Review of Phytopathology·Said A Ghabrial, Nobuhiro Suzuki
Nov 26, 2009·Uirusu·Koki TaniguchiMasanori Kugita
Jul 16, 2011·Annual Review of Microbiology·Eckard Wimmer, Aniko V Paul
Nov 18, 2009·Expert Opinion on Biological Therapy·Diana J M Van Den WollenbergRob C Hoeben
Jan 1, 2014·Uirusu·Satoshi Komoto, Koki Taniguchi
Jul 4, 2012·Current Opinion in Virology·Koki Taniguchi, Satoshi Komoto
Jul 6, 2012·Current Opinion in Virology·Mary K Estes, Ulrich Desselberger
Sep 17, 2014·Genes & Genetic Systems·Yoshiyuki Suzuki
Mar 5, 2010·The Journal of General Virology·R ContinO R Burrone
Jul 18, 2008·The Journal of General Virology·Polly Roy
Mar 25, 2015·Nucleic Acids Research·Ana Eusebio-Cope, Nobuhiro Suzuki
Aug 12, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Satoshi KomotoKoki Taniguchi
Sep 22, 2006·Virology·Michael R Roner, Bradley G Steele
Jul 13, 2014·Virus Research·Ulrich Desselberger
Jan 1, 2014·Uirusu·Takeshi Kobayashi
Jun 16, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Satoshi KomotoKoki Taniguchi
Jun 15, 2016·Annual Review of Phytopathology·Taiyun Wei, Yi Li
Oct 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Takeshi Tsugawa, Hiroyuki Tsutsumi

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