PMID: 9440516Jan 24, 1998Paper

Reverse gyrase from the hyperthermophilic bacterium Thermotoga maritima: properties and gene structure

Journal of Bacteriology
C Bouthier de la TourM Duguet

Abstract

The hyperthermophilic bacterium Thermotoga maritima MSB8 possesses a reverse gyrase whose enzymatic properties are very similar to those of archaeal reverse gyrases. It catalyzes the positive supercoiling of the DNA in an Mg2+- and ATP-dependent process. Its optimal temperature of activity is around 90 degrees C, and it is highly thermostable. We have cloned and DNA sequenced the corresponding gene (T. maritima topR). This is the first report describing the analysis of a gene encoding a reverse gyrase in bacteria. The T. maritima topR gene codes for a protein of 1,104 amino acids with a deduced molecular weight of 128,259, a value in agreement with that estimated from the denaturing gel electrophoresis of the purified enzyme. Like its archaeal homologs, the T. maritima reverse gyrase exhibits helicase and topoisomerase domains, and its sequence matches very well the consensus sequence for six reverse gyrases now available. Phylogenetic analysis shows that all reverse gyrases, including the T. maritima enzyme, form a very homogeneous group, distinct from the type I 5' topoisomerases of the TopA subfamily, for which we have previously isolated a representative gene in T. maritima (topA). The coexistence of these two distinct gene...Continue Reading

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Citations

Jun 8, 2001·Annual Review of Biochemistry·J J Champoux
Apr 5, 2000·The Journal of Biological Chemistry·A C DéclaisM Duguet
Jul 12, 2014·Nucleic Acids Research·Pavel Lulchev, Dagmar Klostermeier
Jul 11, 2008·The Journal of Biological Chemistry·Claire Bouthier de la TourMichel Duguet
Jun 27, 2009·The Journal of Microbiology·Michelle Heine, Sathees B C Chandra
Mar 26, 2011·Journal of Molecular Biology·Anna BizardMarc Nadal
Oct 30, 1998·Biochemical and Biophysical Research Communications·A Ahumada, Y C Tse-Dinh
Sep 28, 1998·Biochimica Et Biophysica Acta·Y C Tse-Dinh
Jun 15, 2007·Journal of Molecular Biology·Stefan P Jungblut, Dagmar Klostermeier

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