PMID: 8972852Dec 1, 1996Paper

Reverse gyrase gene from Sulfolobus shibatae B12: gene structure, transcription unit and comparative sequence analysis of the two domains

Nucleic Acids Research
C JaxelMarc Nadal

Abstract

We cloned and sequenced a DNA fragment from the thermophilic archaeal strain Sulfolobus shibatae B12 that includes the gene topR encoding the reverse gyrase. The RNA of the reverse gyrase gene was characterized indicating that the topR gene is fully functional in vivo. We showed by primer extension analysis that transcription of topR initiates 28 bp downstream from a consensus A-box promoter. In order to understand how this particular type I DNA topoisomerase introduces positive superturns into the DNA, we compared the amino acid sequence of reverse gyrase from S.shibatae with the two other known reverse gyrases. This comparison indicates a common organization of these proteins: the carboxy-terminal domain is related to the type I-5' topoisomerase family while the amino-terminal domain possesses some motifs of proteins described as RNA or DNA helicases. By using local alignments, we showed that (i) reverse gyrases constitute a new and rather homogenous group within the type I-5' DNA topoisomerase family; (ii) a careful sequence analysis of the amino-terminal domain allows us to relate the presence of some motifs with an ATP binding and hydrolysis reaction coupled to a DNA binding and unwinding activity.

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Citations

Sep 28, 1998·Biochimica Et Biophysica Acta·Y C Tse-Dinh
Mar 26, 1999·European Journal of Biochemistry·C JaxelM Nadal
Feb 2, 2002·The EMBO Journal·A Chapin Rodríguez, Daniela Stock
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Sep 27, 2014·International Journal of Molecular Sciences·Valeria VisoneMaria Ciaramella
Mar 26, 2011·Journal of Molecular Biology·Anna BizardMarc Nadal
Feb 13, 2007·Biochimie·Patrick ForterreMarie-Claude Serre
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Jul 12, 2014·Nucleic Acids Research·Pavel Lulchev, Dagmar Klostermeier
Dec 19, 2017·Nucleic Acids Research·Anna H BizardMarc Nadal
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May 9, 2021·Nucleic Acids Research·Dian SpakmanGraeme A King
Jun 12, 2021·Frontiers in Microbiology·Florence GarnierMarc Nadal

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