PMID: 2507379Oct 1, 1989Paper

Reverse hemolytic plaque assay study of luteinizing and follicle-stimulating hormone and thyrotropin secretion in diabetic rat pituitary glands

Diabetes
G L RossiM Varini

Abstract

Numerous studies indicate that an impaired hypothalamopituitary axis plays an important role in reproductive and thyroid disorders in diabetic humans and animal models. Yet, several questions about the pathogenesis of these diabetic complications have not been answered. To evaluate the basal secretion of single gonadotrophs and thyrotrophs in vitro, uncultured pituitary cells from control rats and 1-mo streptozocin-induced diabetic (STZ-D) rats were studied with a reverse hemolytic plaque assay and morphometry. After light-microscopy immunocytochemistry for gonadotropin and thyrotropin (TSH), we recorded the ratio of plaque-forming to non-plaque-forming cells. The area of plaques produced by luteinizing hormone (LH), follicle-stimulating hormone (FSH), and TSH cells and the area of plaque-forming and non-plaque-forming cells were clearly smaller in diabetic than control rats. The plaque area, however, was more severely reduced than the cell area. The percentage of LH-, FSH-, and TSH-immunoreactive plaque-forming cells was greatly decreased in diabetic compared with control animals. In conclusion, our findings demonstrate that the LH-, FSH-, and TSH-secreting cells of diabetic rats released less hormone and were less numerous th...Continue Reading

Citations

Dec 15, 1993·Mechanisms of Ageing and Development·G L RossiR Neiger
Sep 1, 1994·Anatomia, histologia, embryologia·R SarriaF Doñate Oliver
Jan 1, 1990·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·D K TontisG L Rossi
Apr 1, 1997·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·G E BestettiG L Rossi

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