Nov 1, 2008

Reverse painting of microdissected chromosome 19 markers in ovarian carcinoma identifies a complex rearrangement map

Genes, Chromosomes & Cancer
Francesca MicciSverre Heim

Abstract

Alterations of chromosome bands 19p13 and 19q13 in the form of added extra material of unknown origin are among the most frequent cytogenetic changes in ovarian carcinomas. To investigate the chromosomal composition of the 19p+ and/or 19q+ markers, we selected for examination 26 ovarian carcinomas which by G-banding had one to four 19p+ and/or 19q+, in total 37 markers. These cases were then subjected to chromosomal microdissection with subsequent reverse painting, which gave informative results on 29 markers. The breakpoints on chromosome 19 were located in both the short (p; n = 15) and the long (q; n = 10) arms, as well as in the centromeric (n = 2) and pericentromeric (n = 6) region. The analysis showed that many chromosomes added material to chromosome 19, but the chromosome arms 11q, 21q, and 22q were particularly common donors. Homogeneously staining regions (hsr) were seen in only three markers, in all of them consisting of 19p material. Eighteen markers were derived from an unbalanced translocation involving chromosome 19. In five markers, chromosome 19 was rearranged with two chromosomes. The most complex marker showed chromosome 19 rearranged with three other chromosomes, i.e., X, 13, and 16. In five markers, all of ...Continue Reading

  • References17
  • Citations16
  • References17
  • Citations16

Citations

Mentioned in this Paper

Ovarian Diseases
Chromosome Banding
Cytogenetics
Epithelial Ovarian Cancer
Unbalanced Translocation
G-Banding
Ovarian Neoplasm
Ovary
Genome Mapping
Ovarian Carcinoma

Related Feeds

Adenocarcinoma, Clear Cell

Clear Cell Adenocarcinoma is a tumor that arises in the female genital tract and is characterized by cells that appear clear under the microscope. Discover the latest research here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Robert F PlaceRajvir Dahiya
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Eun Ji NamSunghoon Kim
Nature Reviews. Cancer
Aurora Esquela-Kerscher, Frank J Slack
© 2020 Meta ULC. All rights reserved