Reverse Phase Protein Microarrays

Methods in Molecular Biology
Elisa BaldelliMariaelena Pierobon

Abstract

While genes and RNA encode information about cellular status, proteins are considered the engine of the cellular machine, as they are the effective elements that drive all cellular functions including proliferation, migration, differentiation, and apoptosis. Consequently, investigations of the cellular protein network are considered a fundamental tool for understanding cellular functions.Alteration of the cellular homeostasis driven by elaborate intra- and extracellular interactions has become one of the most studied fields in the era of personalized medicine and targeted therapy. Increasing interest has been focused on developing and improving proteomic technologies that are suitable for analysis of clinical samples. In this context, reverse-phase protein microarrays (RPPA) is a sensitive, quantitative, high-throughput immunoassay for protein analyses of tissue samples, cells, and body fluids.RPPA is well suited for broad proteomic profiling and is capable of capturing protein activation as well as biochemical reactions such as phosphorylation, glycosylation, ubiquitination, protein cleavage, and conformational alterations across hundreds of samples using a limited amount of biological material. For these reasons, RPPA represe...Continue Reading

Citations

Aug 31, 2019·Expert Review of Proteomics·Huan QiSheng-Ce Tao
Mar 6, 2019·Nature Medicine·Kirsten L BryantChanning J Der
Jun 6, 2019·Molecular Cancer Research : MCR·Erika ParasidoChris Albanese
Dec 16, 2020·Molecular Oncology·Xiaoliang ZhaoGiuseppe Giaccone
Oct 9, 2021·Journal for Immunotherapy of Cancer·Elisa BaldelliMariaelena Pierobon
Oct 13, 2021·Molecular Cancer Research : MCR·Aisha NaeemChris Albanese

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