Reverse transcriptase activity innate to DNA polymerase I and DNA topoisomerase I proteins of Streptomyces telomere complex

Proceedings of the National Academy of Sciences of the United States of America
K Bao, S N Cohen

Abstract

Replication of Streptomyces linear chromosomes and plasmids proceeds bidirectionally from a central origin, leaving recessed 5' termini that are extended by a telomere binding complex. This complex contains both a telomere-protecting terminal protein (Tpg) and a telomere-associated protein that interacts with Tpg and the DNA ends of linear Streptomyces replicons. By using histidine-tagged telomere-associated protein (Tap) as a scaffold, we identified DNA polymerase (PolA) and topoisomerase I (TopA) proteins as other components of the Streptomyces telomere complex. Biochemical characterization of these proteins indicated that both PolA and TopA exhibit highly efficient reverse transcriptase (RT) activity in addition to their predicted functions. Although RT activity innate to other DNA-dependent DNA polymerases has been observed previously, its occurrence in a topoisomerase is unprecedented. Deletion mapping and sequence analysis showed that the RT activity of Streptomcyces TopA resides in a peptide region containing motifs that are absent from most bacterial topoisomerases but are highly conserved in a novel subfamily of eubacterial topoisomerases found largely in Actinobacteria. Within one of these motifs, and essential to the...Continue Reading

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Citations

Oct 25, 2013·Frontiers of Medicine·Yiming LuJing Ye
Sep 28, 2006·Natural Product Reports·Zixin Deng, Linquan Bai
Apr 24, 2013·Molecular Biology and Evolution·Thomas W SchoenfeldBrian P Hedlund
Oct 24, 2006·Applied and Environmental Microbiology·Chien-Chin YangCarton W Chen
Nov 13, 2007·Journal of Bacteriology·Tzu-Wen Huang, Carton W Chen
Sep 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Neal F Lue, Sulin Jiang
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alfredo VillasanteMaría Méndez-Lago
Jan 18, 2016·Molecular Cell·Delphine Benarroch-PopivkerMarie-Josèphe Giraud-Panis
Nov 19, 2005·Research in Microbiology·Jean-Luc Jestin, Sophie Vichier-Guerre
Jun 2, 2014·Nucleic Acids Research·Marcin Jan SzafranDagmara Jakimowicz
Mar 1, 2007·FEMS Microbiology Letters·Alexander StollJohn Cullum
Dec 9, 2008·Journal of Bacteriology·Yuh-ru LinCarton W Chen
Jul 14, 2020·FEMS Microbiology Reviews·Marcin J SzafranMarie A Elliot
Sep 3, 2019·Biologicals : Journal of the International Association of Biological Standardization·Fabien PerugiKlaus Schwamborn

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