PMID: 7544458Jul 25, 1995Paper

Reverse transcriptase and substrate dependence of the RNA hypermutagenesis reaction

Nucleic Acids Research
M A MartínezS Wain-Hobson

Abstract

G-->A hypermutation is a remarkable phenomenon resulting from retroviral reverse transcription in the presence of highly biased dNTP concentrations. Of the three reverse transcriptases (RTases) available, those of human immunodeficiency virus type 1 (HIV-1), avian myeloblastosis virus (AMV) and Moloney murine leukemia virus (MoMLV), the HIV-1 enzyme showed the greatest sensitivity to biased [dCTP]/[dTTP] ratios. The HIV-1 RTase was able to discriminate between dUTP, dITP and the four DNA precursors and was insensitive to pH. There was little preference for nucleotide contexts. A few exceptionally modified sequences were found presumably resulting from G-->A hypermutation and multiple strand transfer. This particular predilection of the HIV-1 and, by extrapolation, the lentiviral RTases towards G-->A hypermutation suggests that the phenomenon may have contributed to the remarkably elevated A content of these retroviral genomes.

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Citations

Oct 15, 2003·Trends in Molecular Medicine·Jean-Pierre VartanianSimon Wain-Hobson
Oct 12, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Elena CarideAmilcar Tanuri
Aug 10, 1997·AIDS Research and Human Retroviruses·R CheynierS Wain-Hobson
Jul 15, 1996·Nucleic Acids Research·J P VartanianS Wain-Hobson
Jan 15, 1996·Nucleic Acids Research·V PezoS Wain-Hobson
Aug 13, 2010·Journal of Virology·Eri MiyagiKlaus Strebel
Nov 8, 2012·Retrovirology·Antoinette C van der Kuyl, Ben Berkhout
Dec 18, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Elena Herrera-CarrilloBen Berkhout
Jan 25, 2005·Journal of Virological Methods·K J Huang, D P Wooley
Aug 31, 1999·The Journal of General Virology·J P VartanianA Meyerhans
Jul 11, 1997·Journal of Molecular Biology·J P VartanianS Wain-Hobson
Aug 18, 2021·Emerging Microbes & Infections·Josep GregoriJosep Quer

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