Reverse transcriptase polymerase chain reaction (RT-PCR) detection of HLP gene expression in Candida glabrata and its possible role in in vitro haemolysin production

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
G LuoG Tang

Abstract

Although haemolysins are known to be putative virulence factors contributing to pathogenicity in Candida species, the haemolytic activity of Candida glabrata and its genetic expression is ill understood at present. Thus, we studied a total of 34 Candida glabrata isolates for their in vitro haemolytic activity using a previously described plate assay system. The mRNA expression of HLP, a putative haemolysin gene, of these isolates was also evaluated using a semi-quantitative, non-competitive RT-PCR assay. All 34 C. glabrata isolates exhibited both partial (alpha) and complete (beta) haemolytic activity to varying degrees. In parallel with the haemolytic activity, all isolates were also positive for HLP mRNA expression. The expression levels of HLP mRNA (as relative units) ranged from 1.01 to 1.82, with a mean value of 1.32. On regression analysis of latter values and the haemolytic activity (in terms of the dimension of the haemolytic zone in the plate assay) of the C. glabrata isolates a highly significant positive correlation was noted (r=0.759, p<0.0001). Taken together, our data illustrate not only the phenotypic characteristics of haemolysin(s) and HLP expression of a battery of C. glabrata clinical isolates, but also, for ...Continue Reading

References

Jan 1, 1981·Archives of Oral Biology·L P Samaranayake, T W MacFarlane
Nov 1, 1981·Reviews of Infectious Diseases·J J Bullen
Feb 1, 1995·Infection and Immunity·M BélangerM Jacques
Nov 1, 1994·Infection and Immunity·J M MannsH R Buckley
Nov 1, 1993·Biochemical Society Transactions·C A Hitchcock
Sep 1, 1993·Antimicrobial Agents and Chemotherapy·C A HitchcockD W Warnock
Oct 24, 1998·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·H MustafaR F Bishop
Jul 28, 2001·Journal of Clinical Microbiology·G LuoJ Y Yau

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Citations

Jul 11, 2006·Folia Microbiologica·A Bialková, J Subík
Dec 6, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M S Moreira-OliveiraM L Moretti
Apr 22, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John F FisherCheryl A Newman
Nov 20, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C F RodriguesM Henriques
Dec 23, 2015·Critical Reviews in Microbiology·Bruna GonçalvesSónia Silva
Mar 18, 2011·Trends in Microbiology·Sónia SilvaJoana Azeredo
Dec 21, 2012·Cellular Microbiology·Sascha Brunke, Bernhard Hube
May 1, 2011·Journal of Investigative and Clinical Dentistry·Yuthika Hemamala SamaranayakeLakshman Perera Samaranayake
Sep 25, 2012·Journal of Medical Microbiology·Y H SamaranayakeL P Samaranayake
Aug 25, 2015·BMC Microbiology·Lei WanJincai Zhang
Apr 17, 2015·Journal of Biomedical Science·Marcia Cristina FurlanetoLuciana Furlaneto-Maia
Jan 18, 2018·Folia Microbiologica·Márcia C FurlanetoLuciana Furlaneto-Maia
Jun 24, 2017·Frontiers in Microbiology·Franziska GerwienBernhard Hube
Oct 31, 2017·Frontiers in Microbiology·Diana L Zuza-AlvesGuilherme M Chaves

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