Reverse transcriptase polymerase chain reaction for detecting Ewing's sarcoma in archival fine needle aspiration biopsies

Acta Cytologica
T SchlottM Droese

Abstract

Previous studies on pediatric soft tissue sarcomas have demonstrated a chromosomal 11;22 (q24;q12) translocation in Ewing's sarcoma (ES) and peripheral primitive neuroectodermal tumors that appears to be a unique oncogenic marker. To investigate the usefulness of reverse transcriptase polymerase chain reaction (RT-PCR) as an ancillary method for cytodiagnosis, we tested archival aspirates derived from ES patients to establish whether any beta-actin RNA expression or tumor-specific EWS/FLI-1 gene translocation had occurred. Sixteen skeletal specimens aspirated 10-15 years earlier from patients with cytologic and histologic diagnoses of ES were prepared for PCR. The amplification products were sequenced. Amplifiable RNA was detected in smears from 12 patients by beta-actin RT-PCR. Seven aspirates from beta-actin-positive patients showed ES-specific genomic translocation (58%). Sequence analysis of the resulting PCR fragments revealed EWS/FLI-1 fusion transcriptions of varying length. When RNA was retrievable, RT-PCR applied to routinely stained aspirate smears was a highly specific method in differential diagnosis of ES.

Citations

Feb 28, 2003·Cancer Genetics and Cytogenetics·A Martínez-RamírezJ Benítez
Dec 31, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R D RileyI J Lewis
Mar 25, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Scott E KilpatrickMargaret L Gulley
Aug 11, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christopher H EvansJames W Wells
Dec 9, 2000·Biochemical and Biophysical Research Communications·K NibuJ L Rothstein

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