PMID: 7544885Aug 11, 1995Paper

Reverse transcriptase reads through a 2'-5'linkage and a 2'-thiophosphate in a template

Nucleic Acids Research
J R LorschJ W Szostak

Abstract

Avian myeloblastosis virus and Maloney murine leukemia virus RNase H-reverse transcriptases pause when they encounter a 2'-5' linkage or a 2'-thiophosphate in their template RNAs, but eventually read through these backbone modifications. Both reverse transcriptases pause after the 2'-5' linkage but before the 2'-thiophosphate. These results suggest that in the absence of precise information concerning the behavior of a given reverse transcriptase with respect to a particular lesion or modification, caution should be exercised in the interpretation of primer extension data that is being used to determine the existence of, or map the position of, a crosslink, site of chemical modification or non-standard linkage in an RNA template.

Citations

Apr 4, 2001·The EMBO Journal·H SaitoH Suga
Jul 12, 2002·The EMBO Journal·Jörg Vogel, Thomas Börner
May 15, 1997·Nucleic Acids Research·J VogelT Börner
May 24, 2012·Nucleic Acids Research·Elisa BiondiDonald H Burke
Nov 12, 2003·Journal of Virology·Laura A SalemThomas M Menees
Dec 20, 2012·RNA·William R JeckNorman E Sharpless
May 27, 2009·International Journal of Molecular Sciences·Sergey Y NechaevMarina A Zenkova
May 9, 2001·Biochemical and Biophysical Research Communications·B J PremrajN Yathindra
Sep 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Natalia G StarostinaMichael P Terns
Jul 31, 2014·Proceedings of the National Academy of Sciences of the United States of America·Liang XuDong Wang
Apr 24, 2013·Nature Chemistry·Aaron E EngelhartJack W Szostak
May 29, 2014·International Journal of Molecular Sciences·Hitoshi Suzuki, Toshifumi Tsukahara
Nov 4, 2015·Angewandte Chemie·Christopher CozensPhilipp Holliger
Oct 22, 2005·Angewandte Chemie·Michael Oberhuber, Gerald F Joyce
Nov 19, 2015·Scientific Reports·Ulrike Pfreundt, Wolfgang R Hess
Aug 21, 2001·Molecular Cell·H L MurrayK A Jarrell
Feb 28, 1998·Chemistry & Biology·A Jenne, M Famulok
Aug 1, 1997·Chemistry & Biology·A J Hager, J W Szostak
Jul 17, 2014·Nature Methods·Nathan A SiegfriedKevin M Weeks
Oct 27, 2018·RNA·Jessica Döring, Thomas Hurek
May 20, 2020·Nucleic Acids Research·Daniel DuzdevichJack W Szostak
Aug 5, 2006·The Journal of Biological Chemistry·María Dolores Molina-SánchezNicolás Toro
Jul 23, 2011·Nature Chemistry·Christopher DeckClemens Richert
Jan 18, 2019·Nature·Jeffrey T MorganDavid P Bartel
Feb 13, 2021·Methods : a Companion to Methods in Enzymology·Shasha ShiRui Zhao
Apr 11, 2021·Methods : a Companion to Methods in Enzymology·Amanda Yunn Ee Ng, Jun Wei Pek
Jun 23, 2021·Nucleic Acids Research·Félix LaRoche-JohnstonBenoit Cousineau
Mar 27, 2019·Journal of the American Chemical Society·Abe D PressmanIrene A Chen

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