Reverse transcriptase sequence of paired isolates of cerebrospinal fluid and blood from patients infected with human immunodeficiency virus type 1 during zidovudine treatment.

Journal of Clinical Microbiology
M Di StefanoF Chiodi

Abstract

Human immunodeficiency virus type 1 (HIV-1) isolates obtained from the blood of patients undergoing treatment with 3'-azido-3'-deoxythymidine (zidovudine [AZT]) show a decreased sensitivity to the drug in vitro. The aim of the present study was to determine if HIV-1 variants resistant to AZT are present also in the brain compartment. We selected sequential HIV-1 isolates from the blood and the cerebrospinal fluid (CSF) of six patients with HIV-1 infection undergoing AZT therapy for a time varying between 1 and 3 years. The isolates were used to infect peripheral blood mononuclear cell cultures which were used to prepare viral DNA. The viral DNA was amplified by PCR and then directly sequenced. Analysis of the reverse transcriptase (RT) sequence of the isolates from the CSF during therapy demonstrated that CSF-resistant isolates are characterized by the same mutations documented in resistant isolates from the blood compartment. Isolates obtained from one patient (patient 3) showed the same two mutations (codons 70 and 215) in blood and CSF, whereas isolates obtained from an additional four patients presented a different pattern of mutations in the two compartments. We also analyzed the degree of amino acid homology between RT se...Continue Reading

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Citations

Apr 3, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·M GisslénB Svennerholm
Jul 31, 1999·Journal of Acquired Immune Deficiency Syndromes : JAIDS·M GisslénL Hagberg
Mar 14, 2007·Evolution; International Journal of Organic Evolution·Antonio V BorderíaRafael Sanjuán
Aug 21, 2010·Immunologic Research·K Grovit-Ferbas, M E Harris-White
Sep 1, 1996·Infection·A LafeuilladeC Sayada
Jul 29, 2006·Journal of Neuro-AIDS·L HagbergB Svennerholm
Jun 14, 2008·The Journal of Infectious Diseases·Richard W Price, Serena Spudich
Aug 26, 2000·Scandinavian Journal of Infectious Diseases·M GisslénL Hagberg
Apr 1, 1997·The Annals of Pharmacotherapy·S T MeltonS N Ghaemi
Dec 19, 2001·Journal of Medical Virology·Helen L DevereuxMargaret A Johnson
Dec 3, 1999·Journal of the Neurological Sciences·L AnderssonM Gisslén

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