Reverse vaccinology approach identify an Echinococcus granulosus tegumental membrane protein enolase as vaccine candidate.

Parasitology Research
Wenjia GanXuchu Hu

Abstract

Applying reverse vaccinology strategy, we employed a sequence encoding an enolase from Taenia asiatica to search its homolog in the expression sequence tag (EST) database of Echinococcus granulosus and found two EST sequences (Access number: CN653186 and CN649593) of a clone Eg_PSGRS_13B09 from E. granulosus protoscolex full-length cDNA library, which are responding for the 5' and 3' partial cds of E. granulosus enolase, respectively. Primers are designed according to the 5' end and 3'end of the putative encoding sequence to amplify the genomic DNA of E. granulosus strain isolated from sheep in Qinghai province of China by polymerase chain reaction (PCR). A sole product of 1,449 bp in length was obtained, which contains two little introns of 78 bp and 69 bp, respectively. The introns were excised by unsymmetrical PCR with combined flank sequences of introns as primers. The structural, functional, and immunological characteristics of putative amino acid sequence were predicted by bioinformatics analysis. The complete coding sequence was predicted to encode 433 residues and contain a transmembrane region aa(104-124), with the N terminus outside and C terminus inside. The inside part is quite the functional domain. SWISS-MODEL mod...Continue Reading

References

Jun 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K ItoS Torii
Jun 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K ItoS Torii
Sep 30, 1993·Biochemical and Biophysical Research Communications·G J WaineD P McManus
Aug 11, 2001·Cellular and Molecular Life Sciences : CMLS·V Pancholi
Jul 19, 2003·European Journal of Biochemistry·Véronique HannaertPaul A M Michels
Dec 21, 2004·European Journal of Biochemistry·Ipsita Pal-BhowmickGotam K Jarori
Jun 2, 2005·Parasitology Research·T NakadaY Takahashi
Jul 29, 2005·Medical Microbiology and Immunology·Esperanza RodríguezTeresa Gárate
Oct 19, 2005·International Journal for Parasitology·Mara C RosenzvitDonald P McManus
Jul 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marzia M GiulianiMariagrazia Pizza
Oct 13, 2006·International Journal for Parasitology·Marie LabbéPierre Péry
Dec 22, 2006·Expert Review of Vaccines·Hervé TettelinVega Masignani
Feb 6, 2007·Parasitology·M W Lightowlers
Mar 21, 2007·Biochimica Et Biophysica Acta·A KellerJ Ovádi
Mar 27, 2007·Experimental Parasitology·Wilfredo QuiñonesJuan Luis Concepción
May 1, 2007·Drug Discovery Today·Matthew N Davies, Darren R Flower
May 25, 2007·The Lancet Infectious Diseases·Philip S CraigPeter M Schantz
Jul 27, 2007·Parasitology Research·Gilmer VanegasLuisana Avilán
Sep 6, 2007·Infection and Immunity·Ipsita Pal-BhowmickGotam K Jarori
Oct 4, 2007·Molecular Phylogenetics and Evolution·Christopher A DesjardinsCharles Mitter
Feb 23, 2008·Trends in Biotechnology·Sandro VivonaFrancesco Filippini
Jul 22, 2008·Molecular and Biochemical Parasitology·Ricardo Pérez-SánchezAna Oleaga
Sep 13, 2008·EcoHealth·Zhenghuan WangXiaoqing Liu
Mar 21, 2009·Molecular & Cellular Proteomics : MCP·Feng LiuZe-Guang Han
Apr 8, 2009·Expert Review of Vaccines·Isabel Leroux-Roels, Geert Leroux-Roels
May 30, 2009·Blood·Edward F Plow, Riku Das
Jul 4, 2009·Veterinary Research Communications·G Battelli
Aug 22, 2009·Genome Dynamics·H Tettelin
Sep 23, 2009·Microbes and Infection·Michael HolmesSirinart Ananvoranich

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Citations

Aug 29, 2012·Parasitology Research·Myoung-Ro LeeWon-Ja Lee
Apr 17, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Alexandra Schubert-Unkmeir, Myron Christodoulides
Aug 1, 2014·Parasitology Research·Jing ZhangDamin Luo
Jan 3, 2016·Ticks and Tick-borne Diseases·A E Lew-Tabor, M Rodriguez Valle
Apr 17, 2016·Computer Methods and Programs in Biomedicine·Banghua YangYunyuan Zhang
Sep 29, 2015·Research in Veterinary Science·W H LiB Q Fu
Jan 30, 2013·Experimental Parasitology·A M Martínez-IbeasM Y Manga-González
Feb 25, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Raúl J BobesJuan P Laclette
Dec 5, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Varun JaiswalChittaranjan Rout
Jun 2, 2018·Journal of Immunology Research·Minerva Arce-FonsecaJosé Luis Rosales-Encina

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