Reversed activity of mitochondrial adenine nucleotide translocator in ischemia-reperfusion

Transplantation
Andrey BelousRavi S Chari

Abstract

Graft dysfunction as a result of preservation injury remains a major clinical problem in liver transplantation. This is related in part to accumulation of mitochondrial calcium. In an attempt to sustain cell and mitochondrial integrity during ischemia, intramitochondrial F(0)F(1) adenosine triphosphate (ATP) synthase reverses its activity and hydrolyzes ATP to maintain the mitochondrial transmembrane potential (mdeltapsi). It is not known how cytoplasmic ATP becomes available for hydrolysis by this enzyme. The authors hypothesized that mitochondrial adenine nucleotide translocator (ANT) reverses its activity during ischemia, making cytoplasmic ATP available for hydrolysis by F(0)F(1) ATP synthase. Rat livers were perfused with cold University of Wisconsin solution at 4 degrees C (39.2 degrees F)through the portal vein and processed immediately or after 24 hr of cold storage. Mitochondria were separated by differential centrifugation. ATP-dependent mitochondrial calcium-45 (45Ca)2+ uptake was determined after incubation with ATP (5 mM) or adenosine diphosphate (ADP) (5 mM) with or without 15 microM of bongkrekic acid (BA), an ANT blocker; the nonhydrolyzable analog of ATP (adenosine 5'-beta,gamma-imidotriphosphate [AMP-PNP]) ser...Continue Reading

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Citations

Jun 14, 2012·HPB Surgery : a World Journal of Hepatic, Pancreatic and Biliary Surgery·Kilian WeigandMartina Müller
Jun 19, 2007·The Journal of Surgical Research·Christopher D AndersonRavi S Chari
Jul 20, 2004·Journal of Cellular Biochemistry·Andrey BelousRavi S Chari
May 26, 2005·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Christopher D AndersonRavi S Chari
Sep 8, 2010·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Mahmoud Abu-AmaraAlexander Seifalian
Oct 16, 2015·Journal of the Royal Society, Interface·Arunas L Radzvilavicius, Neil W Blackstone
Apr 27, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Clayton D KnoxRavi S Chari
Jan 11, 2005·World Journal of Gastroenterology : WJG·Qi-Ping Lu, Lei Tian
Jun 24, 2006·Journal of Cellular Biochemistry·Andrey E BelousRavi S Chari
Oct 1, 2004·World Journal of Gastroenterology : WJG·Xiao-Shun HeJie-Fu Huang
Mar 25, 2004·World Journal of Gastroenterology : WJG·Xiao-Dong TanMichio Ogawa
Jul 14, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·I B NicoudR S Chari

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