Reversed-phase high-performance liquid chromatography separation of dimethylaminoazobenzene sulfonyl- and dimethylaminoazobenzene thiohydantoin-amino acid derivatives for amino acid analysis and microsequencing studies at the picomole level

Analytical Biochemistry
V StocchiL Cucchiarini

Abstract

A simple and fast reversed-phase high-performance liquid chromatographic method has been developed for the complete separation of 35 dimethylaminoazobenzene sulfonyl (DABS)-amino acids and by-products. This method allows simultaneous determination of primary and secondary amino acids which can be present in protein and peptide hydrolysates and also detects the presence of cysteic acid, S-sulfocysteine, hydroxyproline, taurine, norleucine, cystine, and delta-hydroxylysine. The precolumn derivatization of amino acids with dimethylaminoazobenzene sulfonyl chloride (DABS-Cl) is simple and quick (10 min at 70 degrees C) and allows the complete reaction of primary and secondary amino acids. The separation of the compounds under investigation is achieved in 25 min using a reversed-phase 3-microns Supelcosil LC-18 column at room temperature. The versatility of the proposed method is documented by amino acid determination on protein samples obtained using different hydrolysis techniques (HCl, methane-sulfonic acid, and NaOH), with attention given to the detection of tryptophan in protein samples with high sugar concentration. Furthermore, we have reported the experimental conditions necessary to apply this method to the amino acid analy...Continue Reading

References

Jul 1, 1976·The Biochemical Journal·J Y Chang, E H Creaser
Feb 11, 1977·Journal of Chromatography·J Y Chang, E H Creaser
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J R Benson, P E Hare
Jul 1, 1976·Analytical Chemistry·E BayerR Uhmann
May 1, 1988·Analytical Biochemistry·J Y Chang
Jan 1, 1980·Annual Review of Biochemistry·E Adams, L Frank
Nov 1, 1981·Analytical Biochemistry·J SalnikowB Wittmann-Liebold
Dec 1, 1981·The Biochemical Journal·J Y Chang
Jul 15, 1981·Analytical Biochemistry·Y TapuhiB L Karger

❮ Previous
Next ❯

Citations

Jan 1, 1995·Journal of Inherited Metabolic Disease·J L Johnson, K V Rajagopalan
Sep 30, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·T TeerlinkA Houdijk
Jan 23, 1999·Journal of Chromatography. a·M Fountoulakis, H W Lahm
Apr 29, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·D EuliE Mussini
Jun 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·P A de WitteP M Agostinis
Aug 13, 2003·Plant Physiology·Mario GiordanoJohn A Raven
Sep 28, 2004·Plant Physiology·Mijeong Lee JeongScott A Harding
Oct 27, 2007·Biochimica Et Biophysica Acta·Francesca GubelliniB Andrea Melandri
May 1, 1997·Free Radical Research·G LubecA C Aufderheide
Nov 21, 2008·Journal of Separation Science·Ramón Checa-MorenoLuis Fermín Capitán-Vallvey
Sep 23, 1994·Journal of Chromatography. B, Biomedical Applications·T Teerlink

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.