Reversed Phase HPLC-DAD Profiling of Carotenoids, Chlorophylls and Phenolic Compounds in Adiantum capillus-veneris Leaves

Frontiers in Chemistry
Alam Zeb, Fareed Ullah

Abstract

Adiantum capillus-veneris is important endangered fern species with several medicinal properties. In this study, the leaves samples were extracted and separated using reversed phase HPLC with DAD for carotenoids, chlorophylls and phenolic compounds. Separation of carotenoids and chlorophylls were carried out using a tertiary gradient system of water, MTBE and methanol-water, while a binary gradient system of methanol-water-acetic acid was used for phenolic profiling. Results revealed eight carotenoids, four pheophytins, and two chlorophylls. Lutein (806.0 μg/g), chlorophyll b' (410.0 μg/g), chlorophyll a (162.4 μg/g), 9'-Z-neoxanthin (142.8 μg/g) and all-E-violaxanthin (82.2 μg/g) were present in higher amounts. The relatively high amounts of lutein may be one of the key indicator of beneficial antioxidant properties. The phenolic profile revealed a total of 13 compounds, namely 4-hydroxybenzoic acid, chlorogenic acid, caftaric acid, kaempferol glycosides, p-coumaric acid, rosmarinic acid, 5-caffeoylquinic acid, and quercetin glycosides. Kaempferol-3-sophorotrioside (58.7 mg/g), chlorogenic acid (28.5 mg/g), 5-O-caffeoylquinic acid (18.7 mg/g), coumaric acid (11.2 mg/g), and its derivative (33.1 mg/g) were present in high amoun...Continue Reading

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Citations

Aug 29, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Muhammad Nadeem, Alam Zeb
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lijun SongPengzhan Liu
Mar 7, 2021·Foods·Marcela DvorakovaLenka Langhansova
Mar 18, 2020·Current Drug Discovery Technologies·Ali KashkooeMohammad M Zarshenas

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